Covalent immobilization of α-Galactosidase from Penicillium griseoroseum and its application in Oligosaccharides Hydrolysis

dc.creatorFalkoski, Daniel Luciano
dc.creatorGuimarães, Valéria Monteze
dc.creatorQueiroz, Marisa Vieira de
dc.creatorAraújo, Elza Fernandes de
dc.creatorAlmeida, Maíra Nicolau de
dc.creatorBarros, Everaldo Gonçalves de
dc.creatorRezende, Sebastião Tavares de
dc.date2018-04-25T17:16:35Z
dc.date2018-04-25T17:16:35Z
dc.date2008-10-21
dc.date.accessioned2023-09-27T22:04:33Z
dc.date.available2023-09-27T22:04:33Z
dc.identifier15590291
dc.identifierhttps://doi.org/10.1007/s12010-008-8387-9
dc.identifierhttp://www.locus.ufv.br/handle/123456789/19134
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8970327
dc.descriptionPartially purified α-Galactosidase from Penicillium griseoroseum was immobilized onto modified silica using glutaraldehyde linkages. The effective activity of immobilized enzyme was 33%. Free and immobilized α-galactosidase showed optimal activity at 45 °C and pH values of 5 and 4, respectively. Immobilized α-galactosidase was more stable at higher temperatures and pH values. Immobilized α-galactosidase from P. griseoroseum maintained 100% activity after 24 h of incubation at 40 °C, while free enzyme showed only 32% activity under the same incubation conditions. Defatted soybean flour was treated with free and immobilized α-galactosidase in batch reactors. After 8 h of incubation, stachyose was completely hydrolyzed in both treatments. After 8 h of incubation, 39% and 70% of raffinose was hydrolyzed with free and immobilized α-galactosidase respectively. Immobilized α-galactosidase was reutilized eight times without any decrease in its activity.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherApplied Biochemistry and Biotechnology
dc.relationv. 158, Issue 3, p. 540–551, September 2009
dc.rightsHumana Press
dc.subjectPenicillium griseoroseum
dc.subjectα-Galactosidase
dc.subjectEnzyme immobilization
dc.subjectModified silica
dc.subjectRaffinose oligosaccharides
dc.subjectSoybean products
dc.titleCovalent immobilization of α-Galactosidase from Penicillium griseoroseum and its application in Oligosaccharides Hydrolysis
dc.typeArtigo


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