Development of a method to quantify sucrose in soybean grains

dc.creatorTeixeira, Arlindo I.
dc.creatorRibeiro, Lucas F.
dc.creatorRezende, Sebastião T.
dc.creatorBarros, Everaldo G.
dc.creatorMoreira, Maurílio A.
dc.date2018-08-23T18:07:06Z
dc.date2018-08-23T18:07:06Z
dc.date2012-02-15
dc.date.accessioned2023-09-27T22:04:06Z
dc.date.available2023-09-27T22:04:06Z
dc.identifier0308-8146
dc.identifierhttps://doi.org/10.1016/j.foodchem.2011.07.128
dc.identifierhttp://www.locus.ufv.br/handle/123456789/21413
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8970212
dc.descriptionThe sucrose content of soybean seeds affects the final flavor of soy-derived products. The aim of this work was to develop a simple, low-cost, spectrophotometric method for sucrose quantification in soybean seeds. To achieve this goal, we combined the action of invertase, an enzyme that hydrolyses sucrose into fructose and glucose, with glucose oxidase, an enzyme widely used for glucose quantification. This system was adapted to ELISA plates, making large-scale analyses possible at low cost, with potential application in routine analyses. To validate this method, sucrose content was determined in seeds of 14 soybean cultivars by this new method, as well as by HPLC and the enzymatic method of Stitt. The correlation coefficients were high and significant between the results obtained with the new method and the HPLC method (r = 0.9766) and the Stiff method (0.9461).
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherFood Chemistry
dc.relationDevelopment of a method to quantify sucrose in soybean grains
dc.rightsOpen Access
dc.subjectSucrose quantification
dc.subjectSoybean
dc.subjectEnzymatic method
dc.subjectInvertase
dc.subjectGlucose oxidase
dc.titleDevelopment of a method to quantify sucrose in soybean grains
dc.typeArtigo


Este ítem pertenece a la siguiente institución