Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorde Oliveira, Jonatas Rafael
dc.creatorde Castro, Vinicius Carlos
dc.creatorVilela, Polyana das Graças Figueiredo
dc.creatorCamargo, Samira Esteves Afonso
dc.creatorCarvalho, Cláudio Antonio Talge
dc.creatorJorge, Antonio Olavo Cardoso
dc.creatorde Oliveira, Luciane Dias
dc.date2014-05-27T11:30:09Z
dc.date2016-10-25T18:52:37Z
dc.date2014-05-27T11:30:09Z
dc.date2016-10-25T18:52:37Z
dc.date2013-08-15
dc.date.accessioned2017-04-06T02:34:33Z
dc.date.available2017-04-06T02:34:33Z
dc.identifierBMC Complementary and Alternative Medicine, v. 13.
dc.identifier1472-6882
dc.identifierhttp://hdl.handle.net/11449/76274
dc.identifierhttp://acervodigital.unesp.br/handle/11449/76274
dc.identifier10.1186/1472-6882-13-208
dc.identifierWOS:000323228600001
dc.identifier2-s2.0-84882586629.pdf
dc.identifier2-s2.0-84882586629
dc.identifierhttp://dx.doi.org/10.1186/1472-6882-13-208
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896975
dc.descriptionBackground: With the emergence of strains resistant to conventional antibiotics, it is important to carry studies using alternative methods to control these microorganisms causing important infections, such as the use of products of plant origin that has demonstrated effective antimicrobial activity besides biocompatibility. Therefore, this study aimed to evaluate the antimicrobial activity of plant extracts of Equisetum arvense L., Glycyrrhiza glabra L., Punica granatum L. and Stryphnodendron barbatimam Mart. against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Candida albicans, Candida tropicalis, and Candida glabrata, and to analyze the cytotoxicity of these extracts in cultured murine macrophages (RAW 264.7).Methods: Antimicrobial activity of plant extracts was evaluated by microdilution method based on Clinical and Laboratory Standards Institute (CLSI), M7-A6 and M27-A2 standards. The cytotoxicity of concentrations that eliminated the microorganisms was evaluated by MTT colorimetric method and by quantification of proinflammatory cytokines (IL-1β and TNF-α) using ELISA.Results: In determining the minimum microbicidal concentration, E. arvense L., P. granatum L., and S. barbatimam Mart. extracts at a concentration of 50 mg/mL and G. glabra L. extract at a concentration of 100 mg/mL, were effective against all microorganisms tested. Regarding cell viability, values were 48% for E. arvense L., 76% for P. granatum L, 86% for S. barbatimam Mart. and 79% for G. glabra L. at the same concentrations. About cytokine production after stimulation with the most effective concentrations of the extracts, there was a significant increase of IL-1β in macrophage cultures treated with S. barbatimam Mart. (3.98 pg/mL) and P. granatum L. (7.72 pg/mL) compared to control (2.20 pg/mL) and a significant decrease of TNF-α was observed in cultures treated with G. glabra L. (4.92 pg/mL), S. barbatimam Mart. (0.85 pg/mL), E. arvense L. (0.83 pg/mL), and P. granatum L. (0.00 pg/mL) when compared to control (41.96 pg/mL).Conclusions: All plant extracts were effective against the microorganisms tested. The G. glabra L. extract exhibited least cytotoxicity and the E. arvense L. extract was the most cytotoxic. © 2013 de Oliveira et al.; licensee BioMed Central Ltd.
dc.languageeng
dc.relationBMC Complementary and Alternative Medicine
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCandida albicans
dc.subjectCandida glabrata
dc.subjectCandida tropicalis
dc.subjectEquisetum arvense L
dc.subjectGlycyrrhiza glabra L
dc.subjectPunica granatum L
dc.subjectStaphylococcus aureus
dc.subjectStaphylococcus epidermidis
dc.subjectStreptococcus mutans
dc.subjectStryphnodendron barbatimam Mart
dc.subjectBrazilian plant extract
dc.subjectequisetum arvense extract
dc.subjectGlycyrrhiza glabra extract
dc.subjectinterleukin 1beta
dc.subjectplant extract
dc.subjectpomegranate extract
dc.subjectStryphnodendron barbatimam extract
dc.subjecttumor necrosis factor alpha
dc.subjectunclassified drug
dc.subjectanimal cell
dc.subjectantimicrobial activity
dc.subjectcell viability
dc.subjectcolorimetry
dc.subjectcontrolled study
dc.subjectcytokine production
dc.subjectcytotoxicity
dc.subjectdentistry
dc.subjectenzyme linked immunosorbent assay
dc.subjectmacrophage
dc.subjectmouse
dc.subjectmouth flora
dc.subjectnonhuman
dc.subjectstimulation
dc.subjectAnimals
dc.subjectAnti-Bacterial Agents
dc.subjectBacteria
dc.subjectBrazil
dc.subjectCell Line
dc.subjectCell Survival
dc.subjectMacrophages
dc.subjectMice
dc.subjectPlant Extracts
dc.subjectPlants, Medicinal
dc.subjectStomatognathic Diseases
dc.subjectTumor Necrosis Factor-alpha
dc.titleCytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry
dc.typeOtro


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