Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae

dc.creatorMartins, G.F.
dc.creatorGuedes, B.A.M.
dc.creatorSilva, L.M.
dc.creatorSerrão, J.E.
dc.creatorFortes-Dias, C.L.
dc.creatorRamalho-Ortigão, J.M.
dc.creatorPimenta, P.F.P.
dc.date2017-11-30T10:27:55Z
dc.date2017-11-30T10:27:55Z
dc.date2011-01-20
dc.date.accessioned2023-09-27T22:01:24Z
dc.date.available2023-09-27T22:01:24Z
dc.identifier15323072
dc.identifierhttps://doi.org/10.1016/j.tice.2010.12.003
dc.identifierhttp://www.locus.ufv.br/handle/123456789/14060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8969587
dc.descriptionOenocytes are ectodermic cells that participate in a number of critical physiological roles such as detoxification and lipid storage and metabolism in insects. In light of the lack of information on oenocytes from Aedes aegypti and the potential role of these cells in the biology of this major yellow fever and dengue vector, we developed a protocol to purify and maintain Ae. aegypti pupa oenocytes in primary culture. Ae. aegypti oenocytes were cultured as clustered and as isolated ovoid cells with a smooth surface. Our results demonstrate that these cells remain viable in cell culture for at least two months. We also investigated their morphology in vivo and in vitro using light, confocal, scanning and transmission electron microscopes. This work is the first successful attempt in isolating and maintaining Ae. aegypti oenocytes in culture, and a significant step towards understanding the role of this cell type in this important disease vector. The purification and the development of primary cultures of insect oenocytes will allow future studies of their metabolism in producing and secreting compounds.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherTissue and Cell
dc.relationVolume 43, Issue 2, Pages 83-90, April 2011
dc.rightsOpen Access
dc.subjectAedes aegypti
dc.subjectOenocyte
dc.subjectPrimary culture
dc.subjectMorphology
dc.subjectMosquito
dc.titleIsolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae
dc.typeArtigo


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