Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping

dc.creatorBueno, Rafael Delmond
dc.creatorGod, Pedro Ivo Vieira Good
dc.creatorPrata, Isadora Oliveira
dc.creatorPereira, Pedro Henrique Scarpelli
dc.creatorTeixeira, Arlindo Inês
dc.creatorPiovesan, Newton Deniz
dc.creatorBarros, Everaldo Gonçalves de
dc.date2019-06-13T17:28:51Z
dc.date2019-06-13T17:28:51Z
dc.date2018-07
dc.date.accessioned2023-09-27T22:00:19Z
dc.date.available2023-09-27T22:00:19Z
dc.identifier1984-7033
dc.identifierhttp://dx.doi.org/10.1590/1984-70332018v18n3a37
dc.identifierhttp://locus.ufv.br//handle/123456789/25833
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8969333
dc.descriptionThe development of molecular markers is essential for improvement of soybean cultivars with modified fatty acid content. The objective of this study was to identify and validate SNP markers in candidate genes for fatty acid content in soybean. Six candidate genes (ARAF, PDAT, ABI3, FAD2-1b, FAD3B, and FAD3C) were selected. Alignment of gene sequences identified 25 SNPs and 3 INDELs. TSP primers were used to identify SNP alleles. 259 recombinant inbred lines (RILs) (FA22 / CD219) and 185 F2 progenies (A29 / Tucunaré) were tested for association of SNPs. An SNP for FAD3B was associated with variation in content of linoleic acid (R2 = 5.84%) and linolenic acid (R2 = 6.79%). In FAD3C, an SNP was associated with linoleic and linolenic acids (R2 of 9.21% and 18.51%, respectively). The ABI3 gene was associated with palmitic acid, with R2 = 5.41%. The SNP markers identified will be used in assisted selection for improvement of fatty acid content.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherCrop Breeding and Applied Biotechnology
dc.relationv. 18, n. 3, p. 244- 251, jul.- set. 2018
dc.rightsOpen Access
dc.subjectLow linolenic acid
dc.subjectMAS- marker- assisted selection
dc.subjectSNP- single nucleotide polymorphism
dc.titleAssociation of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping
dc.typeArtigo


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