Morphology and contractility in cardiomyocytes of rats with low exercise performance

dc.creatorQuintão Júnior, Judson Fonseca
dc.creatorNatali, Antônio José
dc.creatorCarneiro Júnior, Miguel Araújo
dc.creatorCastro, Cynthia Aparecida de
dc.creatorDrummond, Lucas Rios
dc.creatorLavorato, Victor Neiva
dc.creatorFelix, Leonardo Bonato
dc.creatorCruz, Jader dos Santos
dc.creatorPrímola-Gomes, Thales Nicolau
dc.date2017-10-30T11:00:21Z
dc.date2017-10-30T11:00:21Z
dc.date2012-01-24
dc.date.accessioned2023-09-27T21:59:11Z
dc.date.available2023-09-27T21:59:11Z
dc.identifier0066-782X
dc.identifierhttp://dx.doi.org/10.1590/S0066-782X2012005000036
dc.identifierhttp://www.locus.ufv.br/handle/123456789/12489
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8969057
dc.descriptionAerobic capacity is essential to physical performance, and low aerobic capacity is related to the triggering of various cardiovascular diseases. To compare the morphology and contractility of isolated rat cardiomyocytes with low performance and standard performance for exercise. Wistar rats with 10 weeks of age underwent a protocol of treadmill running to fatigue, and were divided into two groups: Low Performance (LP) and Standard Performance (SP). Then, the animals were sacrificed, the heart was quickly removed and, by means of enzymatic dissociation, left ventricular cardiomyocytes were isolated. The cell and sarcomeres length and width of cardiomyocytes were measured using an edge detection system. The isolated cardiomyocytes were electrically stimulated at 1 and 3 Hz and cell contraction was measured by registering the change of their length. The cell length was shorter in the LP group (157.2 1.3µm; p < 0.05) compared to SP (161.4 1.3µm), and the same result was observed for the volume of cardiomyocytes (LP, 25.5 0.4. vs. SP, 26.8 ± 0.4 pL; p < 0.05). The time to peak contraction (LP, 116 1 vs. SP 111 2ms) and total relaxation (LP, 143 3 vs. SP 232 3ms) were higher in the LP group. We conclude that left ventricular myocytes of animals with low performance for exercise are smaller than animals with standard performance. In addition to that, they present losses in contractile capacity.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherArquivos Brasileiros de Cardiologia
dc.relationvol.98, n.5, p.431-436, Apr. 2012
dc.rightsOpen Access
dc.subjectMyocytes
dc.subjectCardiac
dc.subjectRats
dc.subjectExercise
dc.subjectFatigue
dc.subjectMyocardial contraction
dc.subjectPhysical conditioning animal
dc.titleMorphology and contractility in cardiomyocytes of rats with low exercise performance
dc.typeArtigo


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