Fat body, hemolymph and ovary routes for delivery of substances to ovary in Melipona quadrifasciata anthidioides: Differences among castes through the use of electron-opaque tracers

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorDa Cruz-Landim, Carminda
dc.creatorRoat, Thaisa Cristina
dc.creatorBerger, Bruno
dc.date2014-05-27T11:30:06Z
dc.date2016-10-25T18:52:01Z
dc.date2014-05-27T11:30:06Z
dc.date2016-10-25T18:52:01Z
dc.date2013-08-01
dc.date.accessioned2017-04-06T02:33:29Z
dc.date.available2017-04-06T02:33:29Z
dc.identifierJournal of Electron Microscopy, v. 62, n. 4, p. 457-466, 2013.
dc.identifier0022-0744
dc.identifier1477-9986
dc.identifierhttp://hdl.handle.net/11449/76151
dc.identifierhttp://acervodigital.unesp.br/handle/11449/76151
dc.identifier10.1093/jmicro/dft018
dc.identifierWOS:000323425200005
dc.identifier2-s2.0-84883288904
dc.identifierhttp://dx.doi.org/10.1093/jmicro/dft018
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896860
dc.descriptionThe yolk protein precursor, vitellogenin (Vg), in bees is synthesized in the fat body trophocytes, delivered to the hemolymph and ultimately absorbed from there during the vitellogenic phase of oocytes in the active ovary. The routes tracing the material exchange that occurs between the trophocytes and the hemolymph, in addition to the transportation from the hemolymph to the ovarian follicles, were marked by alkaline phosphatase and lanthanum nitrate (LN). Active ovaries from nurse workers and physogastric queens, as well as inactive ovaries of virgin queens, were examined by transmission electron microscopy. The LN permitted better visualization of the routes of exchanges between the organs and the hemolymph. Both methods demonstrate the apparent differences between the phases of the ovary and the bee caste. In inactive ovaries of the virgin queens, the routes from the follicular epithelium to the oocyte remain closed; conversely, they are open in active ovaries of the nurse workers and physogastric queens. The differences between the methods and classes of bees are discussed. © The Author 2013. Published by Oxford University Press [on behalf of The Japanese Society of Microscopy]. All rights reserved.
dc.languageeng
dc.relationJournal of Electron Microscopy
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectalkaline phosphatase
dc.subjectfat body
dc.subjectlanthanum nitrate
dc.subjectovaries
dc.subjectstingless bees
dc.subjectvitellogenin
dc.titleFat body, hemolymph and ovary routes for delivery of substances to ovary in Melipona quadrifasciata anthidioides: Differences among castes through the use of electron-opaque tracers
dc.typeOtro


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