The molecular chaperone binding protein BiP prevents leaf dehydration-induced cellular homeostasis disruption

dc.creatorCarvalho, Humberto H.
dc.creatorBrustolini, Otávio J. B.
dc.creatorPimenta, Maiana R.
dc.creatorMendes, Giselle C.
dc.creatorGouveia, Bianca C.
dc.creatorSilva, Priscila A.
dc.creatorSilva, José Cleydson F.
dc.creatorMota, Clenilso S.
dc.creatorSoares-Ramos, Juliana R. L.
dc.creatorFontes, Elizabeth P. B.
dc.date2017-11-08T13:23:24Z
dc.date2017-11-08T13:23:24Z
dc.date2014-01-29
dc.date.accessioned2023-09-27T21:56:38Z
dc.date.available2023-09-27T21:56:38Z
dc.identifier1932-6203
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0086661
dc.identifierhttp://www.locus.ufv.br/handle/123456789/12892
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8968433
dc.descriptionBiP overexpression improves leaf water relations during droughts and delays drought-induced leaf senescence. However, whether BiP controls cellular homeostasis under drought conditions or simply delays dehydration-induced leaf senescence as the primary cause for water stress tolerance remains to be determined. To address this issue, we examined the drought-induced transcriptomes of BiP-overexpressing lines and wild-type (WT) lines under similar leaf water potential (ψw) values. In the WT leaves, a ψw reduction of −1.0 resulted in 1339 up-regulated and 2710 down-regulated genes; in the BiP-overexpressing line 35S::BiP-4, only 334 and 420 genes were induced and repressed, respectively, at a similar leaf ψw = −1.0 MPa. This level of leaf dehydration was low enough to induce a repertory of typical drought-responsive genes in WT leaves but not in 35S::BiP-4 dehydrated leaves. The responders included hormone-related genes, functional and regulatory genes involved in drought protection and senescence-associated genes. The number of differentially expressed genes in the 35S::BiP-4 line approached the wild type number at a leaf ψw = −1.6 MPa. However, N-rich protein (NRP)- mediated cell death signaling genes and unfolded protein response (UPR) genes were induced to a much lower extent in the 35S::BiP-4 line than in the WT even at ψw = −1.6 MPa. The heatmaps for UPR, ERAD (ER-associated degradation protein system), drought-responsive and cell death-associated genes revealed that the leaf transcriptome of 35S::BiP-4 at ψw = −1.0 MPa clustered together with the transcriptome of well-watered leaves and they diverged considerably from the drought-induced transcriptome of the WT (ψw = −1.0, −1.7 and −2.0 MPa) and 35S::BiP-4 leaves at ψw = −1.6 MPa. Taken together, our data revealed that BiP-overexpressing lines requires a much higher level of stress (ψw = −1.6 MPa) to respond to drought than that of WT (ψw = −1.0). Therefore, BiP overexpression maintains cellular homeostasis under water stress conditions and thus ameliorates endogenous osmotic stress.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherPlos One
dc.relationv. 9, n. 1, e86661, Jan. 2014
dc.rightsOpen Access
dc.subjectChaperone
dc.subjectHomeostasis disruption
dc.titleThe molecular chaperone binding protein BiP prevents leaf dehydration-induced cellular homeostasis disruption
dc.typeArtigo


Este ítem pertenece a la siguiente institución