| dc.creator | Queiroz, Marisa Vieira de | |
| dc.creator | Daboussi, Marie-Josée | |
| dc.date | 2017-11-28T13:43:02Z | |
| dc.date | 2017-11-28T13:43:02Z | |
| dc.date | 2002-12-12 | |
| dc.date.accessioned | 2023-09-27T21:56:06Z | |
| dc.date.available | 2023-09-27T21:56:06Z | |
| dc.identifier | 15746968 | |
| dc.identifier | https://doi.org/10.1111/j.1574-6968.2003.tb11535.x | |
| dc.identifier | http://www.locus.ufv.br/handle/123456789/13890 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8968302 | |
| dc.description | An autonomous impala transposon trapped in Fusarium oxysporum by insertion within the niaD gene encoding nitrate reductase was introduced in the genome of the fungus Penicillium griseoroseum, a producer of pectinase enzymes. Through a phenotypic assay, we demonstrate that this element is able to excise from the niaD gene and to reinsert at new genomic positions. As in the original host, impala inserts into a TA site and footprints left by impala excisions are generally 5 bp. The fact that impala is able to transpose in P. griseoroseum
offers the opportunity to develop a gene-tagging system based on this element with the objective to detect and clone genes related in pectinase production. | |
| dc.format | pdf | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | FEMS Microbiology Letters | |
| dc.relation | Volume 218, Issue 2, Pages 317–321, January 2003 | |
| dc.rights | Open Access | |
| dc.subject | Transposon impala | |
| dc.subject | Heterologous transposition | |
| dc.subject | Filamentous fungus | |
| dc.subject | Penicillium griseoroseum | |
| dc.title | Impala, a transposon from Fusarium oxysporum, is active in the genome of Penicillium griseoroseum | |
| dc.type | Artigo | |
