Lipase production by Botryosphaeria ribis EC-01 on soybean and castorbean meals: Optimization, immobilization, and application for biodiesel production

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorAndrade, Milena M.
dc.creatorBarbosa, Aneli M.
dc.creatorBofinger, Matheus R.
dc.creatorDekker, Robert F. H.
dc.creatorMessias, Josana M.
dc.creatorGuedes, Carmen L. B.
dc.creatorZaminelli, Tiago
dc.creatorDe Oliveira, Bruno H.
dc.creatorDe Lima, Valéria M. G.
dc.creatorDall'Antonia, Luiz H.
dc.date2014-05-27T11:30:05Z
dc.date2016-10-25T18:51:55Z
dc.date2014-05-27T11:30:05Z
dc.date2016-10-25T18:51:55Z
dc.date2013-08-01
dc.date.accessioned2017-04-06T02:33:10Z
dc.date.available2017-04-06T02:33:10Z
dc.identifierApplied Biochemistry and Biotechnology, v. 170, n. 7, p. 1792-1806, 2013.
dc.identifier0273-2289
dc.identifier1559-0291
dc.identifierhttp://hdl.handle.net/11449/76115
dc.identifierhttp://acervodigital.unesp.br/handle/11449/76115
dc.identifier10.1007/s12010-013-0309-9
dc.identifierWOS:000322542900020
dc.identifier2-s2.0-84881233696
dc.identifierhttp://dx.doi.org/10.1007/s12010-013-0309-9
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896825
dc.descriptionThe effects of soybean and castorbean meals were evaluated separately, and in combinations at different ratios, as substrates for lipase production by Botryosphaeria ribis EC-01 in submerged fermentation using only distilled water. The addition of glycerol analytical grade (AG) and glycerol crude (CG) to soybean and castorbean meals separately and in combination, were also examined for lipase production. Glycerol-AG increased enzyme production, whereas glycerol-CG decreased it. A 24 factorial design was developed to determine the best concentrations of soybean meal, castorbean meal, glycerol-AG, and KH2PO4 to optimize lipase production by B. ribis EC-01. Soybean meal and glycerol-AG had a significant effect on lipase production, whereas castorbean meal did not. A second treatment (22 factorial design central composite) was developed, and optimal lipase production (4,820 U/g of dry solids content (ds)) was obtained when B. ribis EC-01 was grown on 0.5 % (w/v) soybean meal and 5.2 % (v/v) glycerol in distilled water, which was in agreement with the predicted value (4,892 U/g ds) calculated by the model. The unitary cost of lipase production determined under the optimized conditions developed ranged from US$0.42 to 0.44 based on nutrient costs. The fungal lipase was immobilized onto Celite and showed high thermal stability and was used for transesterification of soybean oil in methanol (1:3) resulting in 36 % of fatty acyl alkyl ester content. The apparent K m and V max were determined and were 1.86 mM and 14.29 μmol min -1 mg-1, respectively. © 2013 Springer Science+Business Media New York.
dc.languageeng
dc.relationApplied Biochemistry and Biotechnology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAgro-industrial residues
dc.subjectCelite
dc.subjectGlycerol
dc.subjectImmobilization
dc.subjectLipase production
dc.subjectResponse surface method
dc.subjectSubmerged fermentation
dc.subjectAgro-industrial residue
dc.subjectFermentation
dc.subjectOptimization
dc.subjectRadioactive waste vitrification
dc.subjectSubstrates
dc.subjectalkyl group
dc.subjectbiodiesel
dc.subjectester
dc.subjectglycerol
dc.subjectinfusorial earth
dc.subjectmethanol
dc.subjectsoybean oil
dc.subjecttriacylglycerol lipase
dc.subjectwater
dc.subjectbean
dc.subjectbiofuel production
dc.subjectBotryosphaeria ribis
dc.subjectcastorbean
dc.subjectenzyme immobilization
dc.subjectenzyme kinetics
dc.subjectenzyme synthesis
dc.subjectfactorial design
dc.subjectfungus
dc.subjectmeal
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectnutrient
dc.subjectpredictive value
dc.subjectresponse surface method
dc.subjectsoybean
dc.subjectsubmerged fermentation
dc.subjectGlycine max
dc.subjectRicinus communis
dc.titleLipase production by Botryosphaeria ribis EC-01 on soybean and castorbean meals: Optimization, immobilization, and application for biodiesel production
dc.typeOtro


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