A new proposed rodent model of chemically induced prostate carcinogenesis: Distinct time-course prostate cancer progression in the dorsolateral and ventral lobes

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGonçalves, Bianca F.
dc.creatorDe Campos, Silvana G.P.
dc.creatorZanetoni, Cristiani
dc.creatorScarano, Wellerson R.
dc.creatorFalleiros Jr., Luiz R.
dc.creatorAmorim, Renée Laufer
dc.creatorGões, Rejane M.
dc.creatorTaboga, Sebastião R.
dc.date2014-05-27T11:30:04Z
dc.date2016-10-25T18:51:53Z
dc.date2014-05-27T11:30:04Z
dc.date2016-10-25T18:51:53Z
dc.date2013-08-01
dc.date.accessioned2017-04-06T02:33:00Z
dc.date.available2017-04-06T02:33:00Z
dc.identifierProstate, v. 73, n. 11, p. 1202-1213, 2013.
dc.identifier0270-4137
dc.identifier1097-0045
dc.identifierhttp://hdl.handle.net/11449/76097
dc.identifierhttp://acervodigital.unesp.br/handle/11449/76097
dc.identifier10.1002/pros.22669
dc.identifierWOS:000330191800007
dc.identifier2-s2.0-84879330767
dc.identifier0000-0002-0970-4288
dc.identifierhttp://dx.doi.org/10.1002/pros.22669
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896807
dc.descriptionBackground. Characterization of novel rodent models for prostate cancer studies requires evaluation of either spontaneous and carcinogen-induced tumors as well as tumor incidence in different prostatic lobes. We propose a new short-term rodent model of chemically induced prostate carcinogenesis in which prostate cancer progression occurs differentially in the dorsolateral and ventral lobes. Methods. Adult gerbils were treated with MNU alone or associated with testosterone for 3 or 6 months of treatment. Tumor incidence, latency, localization, and immunohistochemistry (AR, PCNA, smooth muscle α-actin, p63, MGMT, and E-cadherin) were studied in both lobes. Results. Comparisons between both lobes revealed that lesions developed first in the DL while the VL presented longer tumor latency. However, after 6 months, there was a dramatic increase in tumor multiplicity in the VL, mainly in MNU-treated groups. Lesions clearly progressed from a premalignant to a malignant phenotype over time and tumor latency was decreased by MNU + testosterone administration. Three-dimensional reconstruction of the prostatic complex showed that the DL developed tumors exclusively in the periurethral area and showed intense AR, PCNA, and MGMT immunostaining. Moreover, VL lesions emerged throughout the entire lobe. MNU-induced lesions presented markers indicative of an aggressive phenotype: lack of basal cells, rupture of the smooth muscle cell layer, loss of E-cadherin, and high MGMT staining. Conclusions. There are distinct pathways involved in tumor progression in gerbil prostate lobes. This animal provides a good model for prostate cancer since it allows the investigation of advanced steps of carcinogenesis with shorter latency periods in both lobes. Copyright © 2013 Wiley Periodicals, Inc.
dc.languageeng
dc.relationProstate
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectalkylating agents
dc.subjectgerbil
dc.subjectN-methyl N-nitrosurea
dc.subjectprostate cancer
dc.subjecttestosterone
dc.subjectalpha smooth muscle actin
dc.subjectmethylated DNA protein cysteine methyltransferase
dc.subjectmethylnitrosourea
dc.subjectprotein p63
dc.subjectuvomorulin
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectcancer growth
dc.subjectcancer incidence
dc.subjectcarcinogenesis
dc.subjectdiagnostic test accuracy study
dc.subjectimmunohistochemistry
dc.subjectmale
dc.subjectnonhuman
dc.subjectphenotype
dc.subjectpriority journal
dc.subjectstaining
dc.subjecturethra
dc.subjectAlkylating Agents
dc.subjectAnimals
dc.subjectDisease Models, Animal
dc.subjectDisease Progression
dc.subjectGerbillinae
dc.subjectMale
dc.subjectMethylnitrosourea
dc.subjectProstate
dc.subjectProstatic Neoplasms
dc.subjectTestosterone
dc.subjectTime Factors
dc.titleA new proposed rodent model of chemically induced prostate carcinogenesis: Distinct time-course prostate cancer progression in the dorsolateral and ventral lobes
dc.typeOtro


Este ítem pertenece a la siguiente institución