Preliminary screening of intestinal barrier genes associated with porcine epidemic diarrhea virus infection in pigs

dc.creatorQi, Xiaoyi
dc.creatorXu, Yafei
dc.creatorQin, Weiyun
dc.creatorWang, Haifei
dc.creatorWu, Shenglong
dc.creatorBao, Wenbin
dc.date2022-12-20T18:21:01Z
dc.date2022-12-20T18:21:01Z
dc.date2020-11-18
dc.date.accessioned2023-09-27T21:50:52Z
dc.date.available2023-09-27T21:50:52Z
dc.identifierQi, X.; Xu, Y.; Qin, W.; Wang, H.; Wu, S. and Bao, W. 2020. Preliminary screening of intestinal barrier genes associated with porcine epidemic diarrhea virus infection in pigs. Revista Brasileira de Zootecnia 49:e20200064
dc.identifier1806-9290
dc.identifierhttps://locus.ufv.br//handle/123456789/30279
dc.identifierhttps://doi.org/10.37496/rbz4920200064
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8967017
dc.descriptionTo screen intestinal barrier genes associated with porcine epidemic diarrhea virus (PEDV) infection, in the present study we first detected PEDV-infected piglets (Sus scrofa) with intestinal damage using quantitative real-time PCR (qPCR) and hematoxylin and eosin (HE) staining. Then, we used qPCR to identify expression differences of intestinal barrier genes between the PEDV-infected and control groups. The results showed that the expression levels of most genes were significantly different between the two groups. Hierarchical clustering and correlation analysis were performed for the expression levels of 25 candidate genes to reveal the key gene that may be involved in PEDV resistance. Two important candidate genes, GLP2 (glucagon– like peptide 2) and AQP3 (aquaporin 3), have their expression positively correlated (r = 0.84). We speculated that decreased expression of GLP2 and AQP3 might play an important role in the process of PEDV infection of piglets by reducing the expression of tight junction proteins and disrupting the junctions between intestinal epithelial cells. There may be an underlying biological interaction between the two genes, which together affect the functional integrity of the intestinal barrier
dc.formatapplication/pdf
dc.languageeng
dc.publisherBrazilian Journal of Animal Science
dc.relationR. Bras. Zootec., 49:e20200064, 2020
dc.rightsCreative Commons Attribution License
dc.subjectgene expression
dc.subjectpiglet
dc.subjectquantitative real-time PCR
dc.titlePreliminary screening of intestinal barrier genes associated with porcine epidemic diarrhea virus infection in pigs
dc.typeArtigo


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