First confirmation and characterization of target and non-target site resistance to glyphosate in Palmer amaranth (Amaranthus palmeri) from Mexico

dc.creatorDominguez-Valenzuela, Jose Alfredo
dc.creatorGherekhloo, Javid
dc.creatorFernández-Moreno, Pablo Tomás
dc.creatorCruz-Hipolito, Hugo Enrique
dc.creatorCruz, Ricardo Alcántara-de la
dc.creatorSánchez-González, Eduardo
dc.creatorPrado, Rafael De
dc.date2018-05-08T11:09:15Z
dc.date2018-05-08T11:09:15Z
dc.date2017-03-28
dc.date.accessioned2023-09-27T21:50:02Z
dc.date.available2023-09-27T21:50:02Z
dc.identifier0981-9428
dc.identifierhttps://doi.org/10.1016/j.plaphy.2017.03.022
dc.identifierhttp://www.locus.ufv.br/handle/123456789/19369
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8966818
dc.descriptionFollowing the introduction of glyphosate-resistant (GR)-cotton crops in Mexico, farmers have relied upon glyphosate as being the only herbicide for in-season weed control. Continuous use of glyphosate within the same year and over multiple successive years has resulted in the selection of glyphosate resistance in Palmer amaranth (Amarantus palmeri). Dose-response assays confirmed resistance in seven different accessions. The resistance ratio based on GR50 values (50% growth reduction) varied between 12 and 83. At 1000 μM glyphosate, shikimic acid accumulation in the S-accession was 30- to 2-fold higher at compared to R-accessions. At 96 h after treatment, 35–44% and 61% of applied 14C-glyphosate was taken up by leaves of plants from R- and S-accessions, respectively. At this time, a significantly higher proportion of the glyphosate absorbed remained in the treated leaf of R-plants (55–69%) compared to S-plants (36%). Glyphosate metabolism was low and did not differ between resistant and susceptible plants. Glyphosate was differentially metabolized to AMPA and glyoxylate in plants of R- and S-accessions, although it was low in both accessions (<10%). There were differences in 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme activity by 50% (I50) between R- and S-accessions. However, no significant differences were found in the basal EPSPS activity (μmol inorganic phosphate μg−1 total soluble protein min−1) between R- and S-accessions. A point mutation Pro-106-Ser was evidenced in three accessions. The results confirmed the resistance of Palmer amaranth accessions to glyphosate collected from GR-cotton crops from Mexico. This is the first study demonstrating glyphosate-resistance in Palmer amaranth from Mexico.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherPlant Physiology and Biochemistry
dc.relationVolume 115, Pages 212-218, June 2017
dc.rightsElsevier Masson SAS.
dc.subjectGlyphosate
dc.subjectHerbicide resistance
dc.subjectMetabolism
dc.subjectPalmer amaranth
dc.subjectTranslocation
dc.subjectPro-106
dc.titleFirst confirmation and characterization of target and non-target site resistance to glyphosate in Palmer amaranth (Amaranthus palmeri) from Mexico
dc.typeArtigo


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