Morphometry and expression of MyoD and Myogenin in white and red skeletal muscles of juvenile fish Colossoma macropomum (Cuvier 1818)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorAlves-Costa, Fernanda A.
dc.creatorBarbosa, Cassiane M.
dc.creatorAguiar, Rachel C. M.
dc.creatorMareco, Edson A.
dc.creatorDal-Pai-Silva, Maeli
dc.date2014-05-27T11:29:57Z
dc.date2016-10-25T18:51:09Z
dc.date2014-05-27T11:29:57Z
dc.date2016-10-25T18:51:09Z
dc.date2013-07-10
dc.date.accessioned2017-04-06T02:30:51Z
dc.date.available2017-04-06T02:30:51Z
dc.identifierActa Zoologica.
dc.identifier0001-7272
dc.identifier1463-6395
dc.identifierhttp://hdl.handle.net/11449/75944
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75944
dc.identifier10.1111/azo.12040
dc.identifier2-s2.0-84879773968
dc.identifierhttp://dx.doi.org/10.1111/azo.12040
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896667
dc.descriptionColossoma macropomum, also known as tambaqui, is an economically important fish species, and interesting new studies have been published with the aim of improving the growth of this fish. In this work, we describe the morphometric characteristics, as well as the mRNA and protein expression levels of two myogenic regulatory factors (MRFs)-myod/MyoD and myogenin/Myogenin-in the white and red muscle types of tambaqui. A high proportion of white and red muscle fibers with large diameters suggest a hypertrophic growth process in the skeletal muscle during juvenile stages. Comparisons between muscle types showed that, in red muscle, myogenin transcript levels were significantly higher than those of myod. In contrast, MyoD protein levels were significantly higher than those of Myogenin in red muscle. These results suggest that in red and white muscles of juvenile tambaqui, independent post-transcriptional mechanisms for regulating MyoD and Myogenin expression may exist, which could be differentially activated during muscle growth. Furthermore, these data also suggest that specific control mechanisms may regulate distinct muscle phenotypes. © 2013 The Royal Swedish Academy of Sciences.
dc.languageeng
dc.relationActa Zoologica
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHyperplasia
dc.subjectHypertrophy
dc.subjectMyogenic regulatory factors
dc.subjectQuantitative RT-PCR
dc.subjectSkeletal muscle
dc.titleMorphometry and expression of MyoD and Myogenin in white and red skeletal muscles of juvenile fish Colossoma macropomum (Cuvier 1818)
dc.typeOtro


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