Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae

dc.creatorSoares, Filippe Elias de Freitas
dc.creatorQueiroz, José Humberto de
dc.creatorBraga, Fabio Ribeiro
dc.creatorAraújo, Jackson Victor de
dc.creatorLima, Walter dos Santos
dc.creatorZamprogno, Tatiana Tonini
dc.date2017-10-30T16:43:02Z
dc.date2017-10-30T16:43:02Z
dc.date2014-11-18
dc.date.accessioned2023-09-27T21:46:48Z
dc.date.available2023-09-27T21:46:48Z
dc.identifier1756-0500
dc.identifierhttps://doi.org/10.1186/1756-0500-7-811
dc.identifierhttp://www.locus.ufv.br/handle/123456789/12535
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8966011
dc.descriptionO link do DOI está errado não pode ter escrito periódico capes.
dc.descriptionThe predatory nematophagous fungus Arthrobotrys sinensis (SF53) produces three proteases with nematicidal activity when grown on solid media culture. However, the proteolytic profile produced by this fungus, when grown in liquid culture medium remains unknown. Thus, the objective of this work was to evaluate the production of proteases from nematophagous fungus Arthrobotrys sinensis in liquid medium and its nematicidal activity on first stage larvae of A. vasorum. Proteases were obtained in its crude form, using Whatman no.1 filter paper, followed by centrifugation for 5 min at 10 × g and 4°C. A zymogram was performed with co-polymerized casein in an acrylamide gel as substrate. An in vitro assay to evaluate the nematicidal action of the proteases of A. sinensis (SF53) produced in liquid medium on A. vasorum L1 was conducted. By the analysis of the zymogram, it was observed a single halo at the beginning of digestion of the gel, suggesting that the three proteases of SF53 are produced in an enzymatic complex of large molecular weight. Regarding nematicidal activity, within 24 hours, the proteases produced in liquid medium of A. sinensis (SF53) showed a percentage reduction of 64% on the number of L1 of A. vasorum. In the present work, it is suggested that the three proteases of SF53 are produced in an enzymatic complex and was also demonstrated that these enzymes were effective in destroying A. vasorum L1.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherBMC Research Notes
dc.relation7:811, November 2014
dc.rightsOpen Access
dc.subjectNematophagous fungi
dc.subjectAngiostrongylus vasorum
dc.subjectProtease
dc.titleProteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae
dc.typeArtigo


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