Relevant peptides of Taenia crassiceps for the diagnosis of bovine cysticercosis by immunoblot

dc.creatorSilva, L.F.
dc.creatorPinto, P. S. A.
dc.creatorDucas, C. T. S.
dc.creatorSantos, T. O.
dc.creatorNieto, E. C. A.
dc.creatorPeixoto, R. P. M. G.
dc.date2019-05-27T23:32:56Z
dc.date2019-05-27T23:32:56Z
dc.date2015-05
dc.date.accessioned2023-09-27T21:46:26Z
dc.date.available2023-09-27T21:46:26Z
dc.identifier1678-4162
dc.identifierhttp://dx.doi.org/10.1590/1678-4162-7291
dc.identifierhttp://www.locus.ufv.br/handle/123456789/25537
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8965921
dc.descriptionGiven the limited knowledge about the diagnosis of bovine cysticercosis by immunoblot, the aim of this study was to assess the applicability of this test, identifying key peptides with diagnostic value. Immunoblot assays were performed using total larval antigen of Taenia crassiceps and 60 sera of positive bovines for cysticercosis (30 naturally and 30 experimentally infected with T. saginata eggs), 30 sera of negative bovines for cysticercosis and 30 sera of bovines with other diseases (fascioliasis, hydatidosis and tuberculosis). The peptides of greater diagnostic importance, in descending order of accuracy (%), were as follows: 6-8kDa (90.8%), 129-143kDa (74.2%), 99-105kDa (71.7%) and 14-19kDa (71.1%). Cross- reactions, due to fascioliasis and hydatidosis, were observed in the four intervals of peptides highlighted. The results demonstrate that the total antigen of T. crassiceps has peptides with a high diagnostic potential; therefore, the immunoblot is useful in the diagnosis of bovine cysticercosis. Given the limited knowledge about the diagnosis of bovine cysticercosis by immunoblot, the aim of this study was to assess the applicability of this test, identifying key peptides with diagnostic value. Immunoblot assays were performed using total larval antigen of Taenia crassiceps and 60 sera of positive bovines for cysticercosis (30 naturally and 30 experimentally infected with T. saginata eggs), 30 sera of negative bovines for cysticercosis and 30 sera of bovines with other diseases (fascioliasis, hydatidosis and tuberculosis). The peptides of greater diagnostic importance, in descending order of accuracy (%), were as follows: 6-8kDa (90.8%), 129-143kDa (74.2%), 99-105kDa (71.7%) and 14-19kDa (71.1%). Cross- reactions, due to fascioliasis and hydatidosis, were observed in the four intervals of peptides highlighted. The results demonstrate that the total antigen of T. crassiceps has peptides with a high diagnostic potential; therefore, the immunoblot is useful in the diagnosis of bovine cysticercosis.
dc.descriptionTendo em vista o conhecimento limitado sobre o diagnóstico da cisticercose bovina pelo immunoblot, o objetivo deste trabalho foi avaliar a aplicabilidade desse teste, identificando os principais peptídeos com valor diagnóstico. Foram realizados ensaios de immunoblot, utilizando antígeno total de larva de Taenia crassiceps e 60 soros de bovinos positivos para a cisticercose (30 naturalmente e 30 experimentalmente infectados com ovos de T. saginata), 30 soros de bovinos negativos para a cisticercose e 30 soros de bovinos com outras patologias (fasciolose, hidatidose e tuberculose). Os peptídeos de maior importância diagnóstica, em ordem decrescente de acurácia (%), foram os seguintes: 6-8 kDa (90,8%), 129-143 kDa (74,2%), 99-105 kDa (71,7%) e 14-19 kDa (71,1%). Reações cruzadas, em decorrência da fasciolose e hidatidose, foram observadas nos quatro intervalos de peptídeos ressaltados. Os resultados demonstram que o antígeno total de T. crassiceps possui peptídeos com alto potencial diagnóstico, sendo, portanto, o immunoblot útil no diagnóstico da cisticercose bovina.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherArquivo Brasileiro de Medicina Veterinária e Zootecnia
dc.relationv. 67, n. 3, p. 891-898, mai./ jun. 2015
dc.rightsOpen Access
dc.subjectBovine cysticercosis
dc.subjectDiagnosis
dc.subjectImmunoblot
dc.subjectTaenia crassiceps
dc.subjectPeptides
dc.subjectCisticercose bovina
dc.subjectDiagnóstico
dc.subjectTaenia crassiceps
dc.subjectPeptídeos
dc.titleRelevant peptides of Taenia crassiceps for the diagnosis of bovine cysticercosis by immunoblot
dc.typeArtigo


Este ítem pertenece a la siguiente institución