Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus

dc.creatorAraújo, J.V.
dc.creatorJunghans, T.G.
dc.creatorAlfenas, A.C.
dc.creatorGomes, A.P.S.
dc.date2017-10-09T18:32:34Z
dc.date2017-10-09T18:32:34Z
dc.date1997-06-28
dc.date.accessioned2023-09-27T21:46:22Z
dc.date.available2023-09-27T21:46:22Z
dc.identifier1414-431X
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X1997001000001
dc.identifierhttp://www.locus.ufv.br/handle/123456789/11962
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8965901
dc.descriptionExtraction and isoenzyme analysis of four isolates of rthrobotrys including A. musiformis, A. robusta and A. conoides were conducted. Among the 14 enzymes studied by starch gel electrophoresis, using morpholine-citrate as gel/electrode buffer, the following nine enzymes showed interpretable banding patterns: α-esterase, fumarase, hexokinase, isocitrate dehydrogenase, leucine aminopeptidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphogluco- mutase and phosphoglucoisomerase. All isolates studied displayed typical isoenzyme phenotypes for each species. Two isolates of A. conoides differed in their α-isoesterase banding patterns, but no differences were observed for the other enzymes. The assay was satisfactory for enzyme extraction and resolution of Arthrobotrys and could be used in future taxonomic and genetic studies of this organism.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherBrazilian Journal of Medical and Biological Research
dc.relationvol. 30 n. 10 1149-1152 Oct. 1997
dc.rightsOpen Access
dc.subjectNematophagous fungi
dc.subjectNematode-trapping fungus
dc.subjectPredacious fungi
dc.subjectArthrobotrys
dc.subjectIsoenzymes
dc.subjectElectrophoresis
dc.titleIsoenzyme analysis of Arthrobotrys, a nematode-trapping fungus
dc.typeArtigo


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