Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorRodríguez-Arellanes, Gabriela
dc.creatorde Sousa, Carolina Nascimento
dc.creatorde Medeiros-Muniz, Mauro
dc.creatorRamírez, José A.
dc.creatorPizzini, Cláudia V.
dc.creatorde Abreu-Almeida, Marcos
dc.creatorda Oliveira, Manoel M. Evangelista
dc.creatorFusco-Almeida, Ana-Marisa
dc.creatorVite-Garín, Tania
dc.creatorPitangui, Nayla S.
dc.creatorEstrada-Bárcenas, Daniel A.
dc.creatorGonzález-González, Antonio E.
dc.creatorMendes-Giannini, Maria José Soares
dc.creatorZancopé-Oliveira, Rosely M.
dc.creatorTaylora, Maria-Lucia
dc.date2014-05-27T11:29:50Z
dc.date2016-10-25T18:50:37Z
dc.date2014-05-27T11:29:50Z
dc.date2016-10-25T18:50:37Z
dc.date2013-07-01
dc.date.accessioned2017-04-06T02:29:12Z
dc.date.available2017-04-06T02:29:12Z
dc.identifierEukaryotic Cell, v. 12, n. 7, p. 1033-1038, 2013.
dc.identifier1535-9778
dc.identifierhttp://hdl.handle.net/11449/75822
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75822
dc.identifier10.1128/EC.00012-13
dc.identifierWOS:000320929500009
dc.identifier2-s2.0-84879361625.pdf
dc.identifier2-s2.0-84879361625
dc.identifier0000-0002-8059-0826
dc.identifierhttp://dx.doi.org/10.1128/EC.00012-13
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896554
dc.descriptionThe MAT1-1 and MAT1-2 idiomorphs associated with the MAT1 locus of Histoplasma capsulatum were identified by PCR. A total of 28 fungal isolates, 6 isolates from human clinical samples and 22 isolates from environmental (infected bat and contaminated soil) samples, were studied. Among the 14 isolates from Mexico, 71.4% (95% confidence interval [95% CI], 48.3% to 94.5%) were of the MAT1-2 genotype, whereas 100% of the isolates from Brazil were of the MAT1-1 genotype. Each MAT1 idiomorphic region was sequenced and aligned, using the sequences of the G-217B (+mating type) and G-186AR (-mating type) strains as references. BLASTn analyses of the MAT1-1 and MAT1-2 sequences studied correlated with their respective+ and-mating type genotypes. Trees were generated by the maximum likelihood (ML) method to search for similarity among isolates of each MAT1 idiomorph. All MAT1-1 isolates originated from Brazilian bats formed a well-defined group; three isolates from Mexico, the G-217B strain, and a subgroup encompassing all soil-derived isolates and two clinical isolates from Brazil formed a second group; last, one isolate (EH-696P) from a migratory bat captured in Mexico formed a third group of the MAT1-1 genotype. The MAT1-2 idiomorph formed two groups, one of which included two H. capsulatum isolates from infected bats that were closely related to the G-186AR strain. The other group was formed by two human isolates and six isolates from infected bats. Concatenated ML trees, with internal transcribed spacer 1 (ITS1) -5.8S-ITS2 and MAT1-1 or MAT1-2 sequences, support the relatedness of MAT1-1 or MAT1-2 isolates. H. capsulatum mating types were associated with the geographical origin of the isolates, and all isolates from Brazil correlated with their environmental sources. © 2013, American Society for Microbiology. All Rights Reserved.
dc.languageeng
dc.relationEukaryotic Cell
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleFrequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil
dc.typeOtro


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