Sexual transmission of Toxoplasma gondii in sheep

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorLopes, Welber Daniel Zanetti
dc.creatorRodriguez, Joana D'Ark
dc.creatorSouza, Fernando A.
dc.creatordos Santos, Thais Rabelo
dc.creatordos Santos, Ricardo Silva
dc.creatorRosanese, Walter Matheus
dc.creatorLopes, Werik Renato Zanetti
dc.creatorSakamoto, Cláudio Alessandro
dc.creatorda Costa, Alvimar José
dc.date2014-05-27T11:29:49Z
dc.date2016-10-25T18:50:21Z
dc.date2014-05-27T11:29:49Z
dc.date2016-10-25T18:50:21Z
dc.date2013-07-01
dc.date.accessioned2017-04-06T02:28:46Z
dc.date.available2017-04-06T02:28:46Z
dc.identifierVeterinary Parasitology, v. 195, n. 1-2, p. 47-56, 2013.
dc.identifier0304-4017
dc.identifier1873-2550
dc.identifierhttp://hdl.handle.net/11449/75781
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75781
dc.identifier10.1016/j.vetpar.2012.12.056
dc.identifierWOS:000320736500006
dc.identifier2-s2.0-84878442385
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2012.12.056
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896513
dc.descriptionMale sheep of reproductive age were distributed into three groups: GI, a sheep inoculated (oral) with 2.0×105 oocysts of the P strain of Toxoplasma gondii; GII, a sheep infected (subcutaneous) with 1.0×106 tachyzoites of the RH strain of T. gondii; and GIII, a sheep kept as a control (not infected). After the inoculation of the males, 12 breeding ewes, which were not pregnant and which were serologically negative for reproductive diseases (particularly toxoplasmosis), were distributed into three groups, synchronized, and subsequently exposed to natural mating with previously inoculated males. The distribution was as follows: five ewes that underwent natural mating with the GI male, five ewes that were exposed to natural mating with the GII male, and two ewes that were mated with the non-infected male (control). Serum samples of all the ewes were collected on days -30, -14, -7, -1, and 0 (days before natural mating) and on days 1, 3, 5, 7, 11, 14, and weekly until birth; the presence of serum antibodies against T. gondii was assessed by IFAT. Using a bioassay and PCR, T. gondii was isolated from the semen of the infected reproducing sheep before mating. Following natural mating, 5 of the 12 females displayed antibodies specific for T. gondii; of these animals, two of the ewes underwent natural mating with the male inoculated with oocysts (GI) and three with the male infected with tachyzoites (GII). One of the females that displayed antibodies specific to this coccidian and that underwent natural mating with the GII sheep had a macerated fetus on the 70th day following coverage. Using a bioassay after the birth, it was possible to isolate T. gondii from samples of the pool of tissues from the five females that seroconverted after natural mating and from their respective lambs. Using PCR, the DNA of T. gondii was isolated from the pool of tissues from one and two females exposed to natural mating with the reproductive males infected with the oocysts and tachyzoites, respectively. Using this technique, it was also possible to diagnose the presence of the parasite in the pool of tissues from the lambs of one female that underwent natural mating with the male sheep infected with oocysts. These results demonstrated the sexual transmission of T. gondii in the sheep species with consequent vertical transmission to their lambs. © 2013 Elsevier B.V.
dc.languageeng
dc.relationVeterinary Parasitology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectOvine
dc.subjectSemen
dc.subjectSexual transmission
dc.subjectToxoplasma gondii
dc.subjectDNA
dc.subjectparasite antibody
dc.subjecttoxoplasma gondii antibody
dc.subjectToxoplasma gondii DNA
dc.subjectunclassified drug
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectantibody specificity
dc.subjectbirth
dc.subjectbreeding line
dc.subjectCoccidia
dc.subjectcontrolled study
dc.subjectDNA isolation
dc.subjectewe
dc.subjectfemale
dc.subjectgenital tract infection
dc.subjectinoculation
dc.subjectlamb
dc.subjectmale
dc.subjectmating
dc.subjectnonhuman
dc.subjectoocyst
dc.subjectparasite isolation
dc.subjectparasite transmission
dc.subjectseroconversion
dc.subjectsexual transmission
dc.subjectsexually transmitted disease
dc.subjectsheep disease
dc.subjectsperm
dc.subjectsubcutaneous tissue
dc.subjecttachyzoite
dc.subjecttissue section
dc.subjecttoxoplasmosis
dc.subjectAnimalia
dc.subjectOvis
dc.subjectOvis aries
dc.titleSexual transmission of Toxoplasma gondii in sheep
dc.typeOtro


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