Optimal single-embryo mass spectrometry fingerprinting

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorTata, Alessandra
dc.creatorSudano, Mateus J.
dc.creatorSantos, Vanessa G.
dc.creatorLandim-Alvarenga, Fernanda D.C.
dc.creatorFerreira, Christina R.
dc.creatorEberlin, Marcos N.
dc.date2014-05-27T11:29:48Z
dc.date2016-10-25T18:50:14Z
dc.date2014-05-27T11:29:48Z
dc.date2016-10-25T18:50:14Z
dc.date2013-07-01
dc.date.accessioned2017-04-06T02:28:17Z
dc.date.available2017-04-06T02:28:17Z
dc.identifierJournal of Mass Spectrometry, v. 48, n. 7, p. 844-849, 2013.
dc.identifier1076-5174
dc.identifier1096-9888
dc.identifierhttp://hdl.handle.net/11449/75731
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75731
dc.identifier10.1002/jms.3231
dc.identifierWOS:000321336000011
dc.identifier2-s2.0-84879961536
dc.identifierhttp://dx.doi.org/10.1002/jms.3231
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896466
dc.descriptionIn pre-implantation embryos, lipids play key roles in determining viability, cryopreservation and implantation properties, but often their analysis is analytically challenging because of the few picograms of analytes present in each of them. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) allows obtaining individual phospholipid profiles of these microscopic organisms. This technique is sensitive enough to enable analysis of individual intact embryos and monitoring the changes in membrane lipid composition in the early stages of development serving as screening method for studies of biology and biotechnologies of reproduction. This article introduces an improved, more comprehensive MALDI-MS lipid fingerprinting approach that considerably increases the lipid information obtained from a single embryo. Using bovine embryos as a biological model, we have also tested optimal sample storage and handling conditions before the MALDI-MS analysis. Improved information at the molecular level is provided by the use of a binary matrix that enables phosphatidylcholines, sphingomyelins, phosphatidylserines, phosphatidylinositols and phosphoethanolamines to be detected via MALDI(±)-MS in both the positive and negative ion modes. An optimal MALDI-MS protocol for lipidomic monitoring of a single intact embryo is therefore reported with potential applications in human and animal reproduction, cell development and stem cell research. Copyright © 2013 John Wiley & Sons, Ltd. Copyright © 2013 John Wiley & Sons, Ltd.
dc.languageeng
dc.relationJournal of Mass Spectrometry
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectbinary matrix
dc.subjectembryo
dc.subjectfingerprinting
dc.subjectmatrix-assisted laser desorption-mass spectrometry (MALDI-MS)
dc.subjectphospholipids
dc.subjectBinary matrix
dc.subjectMALDI-MS
dc.subjectMatrix-assisted laser desorption/ionization mass spectrometry
dc.subjectMembrane lipid composition
dc.subjectMicroscopic organisms
dc.subjectPre-implantation embryos
dc.subjectOptimization
dc.subjectPhospholipids
dc.subjectStem cells
dc.subjectMass spectrometry
dc.subjectphosphatidylcholine
dc.subjectphosphatidylinositol
dc.subjectphosphatidylserine
dc.subjectphosphoethanolamine
dc.subjectsphingomyelin
dc.subjectcell maturation
dc.subjectfemale
dc.subjectfertilization in vitro
dc.subjectlipidomics
dc.subjectmass spectrometry
dc.subjectmatrix associated laser desorption ionization mass spectrometry
dc.subjectnonhuman
dc.subjectoocyte
dc.subjectpriority journal
dc.subjectreproduction
dc.subjectstem cell
dc.subjectstorage
dc.titleOptimal single-embryo mass spectrometry fingerprinting
dc.typeOtro


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