Peripheral kappa and delta opioid receptors are involved in the antinociceptive effect of crotalphine in a rat model of cancer pain

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBrigatte, Patricia
dc.creatorKonno, Katsuhiro
dc.creatorGutierrez, Vanessa Pacciari
dc.creatorSampaio, Sandra Coccuzzo
dc.creatorZambelli, Vanessa Olzon
dc.creatorPicolo, Gisele
dc.creatorCuri, Rui
dc.creatorCury, Yara
dc.date2014-05-27T11:29:41Z
dc.date2016-10-25T18:49:49Z
dc.date2014-05-27T11:29:41Z
dc.date2016-10-25T18:49:49Z
dc.date2013-06-17
dc.date.accessioned2017-04-06T02:26:55Z
dc.date.available2017-04-06T02:26:55Z
dc.identifierPharmacology Biochemistry and Behavior, v. 109, p. 1-7.
dc.identifier0091-3057
dc.identifier1873-5177
dc.identifierhttp://hdl.handle.net/11449/75669
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75669
dc.identifier10.1016/j.pbb.2013.04.012
dc.identifier2-s2.0-84878835594
dc.identifierhttp://dx.doi.org/10.1016/j.pbb.2013.04.012
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896407
dc.descriptionCancer pain is an important clinical problem and may not respond satisfactorily to the current analgesic therapy. We have characterized a novel and potent analgesic peptide, crotalphine, from the venom of the South American rattlesnake Crotalus durissus terrificus. In the present work, the antinociceptive effect of crotalphine was evaluated in a rat model of cancer pain induced by intraplantar injection of Walker 256 carcinoma cells. Intraplantar injection of tumor cells caused the development of hyperalgesia and allodynia, detected on day 5 after tumor cell inoculation. Crotalphine (6 μg/kg), administered p.o., blocked both phenomena. The antinociceptive effect was detected 1 h after treatment and lasted for up to 48 h. Intraplantar injection of nor-binaltorphimine (50 g/paw), a selective antagonist of κ-opioid receptors, antagonized the antinociceptive effect of the peptide, whereas N,N-diallyl-Tyr-Aib-Phe-Leu (ICI 174,864, 10 μg/paw), a selective antagonist of δ-opioid receptors, partially reversed this effect. On the other hand, D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr amide (CTOP, 20 g/paw), an antagonist of μ-opioid receptors, did not modify crotalphine-induced antinociception. These data indicate that crotalphine induces a potent and long lasting opioid-mediated antinociception in cancer pain. © 2013 Elsevier Inc.
dc.languageeng
dc.relationPharmacology Biochemistry and Behavior
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAntinociception
dc.subjectCancer pain
dc.subjectCrotalphine
dc.subjectOpioid receptors
dc.subjectWalker 256 carcinoma cells
dc.subjectantinociceptive agent
dc.subjectcrotalphine
dc.subjectdelta opiate receptor
dc.subjectkappa opiate receptor
dc.subjectmorphine
dc.subjectunclassified drug
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectantinociception
dc.subjectcancer growth
dc.subjectcancer pain
dc.subjectdrug effect
dc.subjectdrug efficacy
dc.subjectlocomotion
dc.subjectmale
dc.subjectnonhuman
dc.subjectpain threshold
dc.subjectpriority journal
dc.subjectrat
dc.titlePeripheral kappa and delta opioid receptors are involved in the antinociceptive effect of crotalphine in a rat model of cancer pain
dc.typeOtro


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