| dc.creator | Pereira, José Antonio Marques | |
| dc.creator | Rosa, Paulo De Tarso Vieira E. | |
| dc.creator | Pastore, Glaucia Maria | |
| dc.creator | Santana, Cesar Costapinto | |
| dc.date | 2018-05-16T13:55:59Z | |
| dc.date | 2018-05-16T13:55:59Z | |
| dc.date | 1998-03 | |
| dc.date.accessioned | 2023-09-27T21:38:31Z | |
| dc.date.available | 2023-09-27T21:38:31Z | |
| dc.identifier | 15590291 | |
| dc.identifier | https://doi.org/10.1007/BF02920188 | |
| dc.identifier | http://www.locus.ufv.br/handle/123456789/19616 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8963945 | |
| dc.description | The application of expanded-bed ion-exchange resins allows the elimination of intermediary particulate separation steps like filtration or centrifugation prior to adsorption steps in enzyme-purification processes from crude fermentation broths. This work is concerned with the experimental evaluation data of a process related to the adsorption of an extracellular p-galactosidase from the fungiScopulariopsis. The protein recovery in the ion-exchange resin Accell Plus QMA™ was accomplished using a continuous-monitoring method. The direct adsorption step was followed by a elution step with concentrated NaCl solutions aiming to improve the enzyme-specific activity. Experimental data for fixed and expanded bed were compared | |
| dc.format | pdf | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Applied Biochemistry and Biotechnology | |
| dc.relation | v. 70, Issue 1, p. 779–787, March 1998 | |
| dc.rights | Humana Press Inc. | |
| dc.subject | Extracellular p-galactosidase | |
| dc.subject | Adsorption | |
| dc.subject | Expanded bed | |
| dc.subject | Enzyme recovery | |
| dc.title | Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase | |
| dc.type | Artigo | |
