Corneal Absorption of a New Riboflavin-Nanostructured System for Transepithelial Collagen Cross-Linking

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBottos, Katia M.
dc.creatorOliveira, Anselmo Gomes de
dc.creatorBersanetti, Patrícia A.
dc.creatorNogueira, Regina F.
dc.creatorLima-Filho, Acácio A. S.
dc.creatorCardillo, José A.
dc.creatorSchor, Paulo
dc.creatorChamon, Wallace
dc.date2014-05-27T11:29:40Z
dc.date2016-10-25T18:49:46Z
dc.date2014-05-27T11:29:40Z
dc.date2016-10-25T18:49:46Z
dc.date2013-06-13
dc.date.accessioned2017-04-06T02:26:43Z
dc.date.available2017-04-06T02:26:43Z
dc.identifierPLoS ONE, v. 8, n. 6, 2013.
dc.identifier1932-6203
dc.identifierhttp://hdl.handle.net/11449/75651
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75651
dc.identifier10.1371/journal.pone.0066408
dc.identifierWOS:000321038800025
dc.identifier2-s2.0-84879942078.pdf
dc.identifier2-s2.0-84879942078
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0066408
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896389
dc.descriptionCorneal collagen cross-linking (CXL) has been described as a promising therapy for keratoconus. According to standard CXL protocol, epithelium should be debrided before treatment to allow penetration of riboflavin into the corneal stroma. However, removal of the epithelium can increase procedure risks. In this study we aim to evaluate stromal penetration of a biocompatible riboflavin-based nanoemulsion system (riboflavin-5-phosphate and riboflavin-base) in rabbit corneas with intact epithelium. Two riboflavin nanoemulsions were developed. Transmittance and absorption coefficient were measured on corneas with intact epithelia after 30, 60, 120, 180, and 240 minutes following exposure to either the nanoemulsions or standard 0.1% or 1% riboflavin-dextran solutions. For the nanoemulsions, the epithelium was removed after measurements to assure that the riboflavin had passed through the hydrophobic epithelium and retained within the stroma. Results were compared to de-epithelialized corneas exposed to 0.1% riboflavin solution and to the same riboflavin nanoemulsions for 30 minutes (standard protocol). Mean transmittance and absorption measured in epithelialized corneas receiving the standard 0.1% riboflavin solution did not reach the levels found on the debrided corneas using the standard technique. Neither increasing the time of exposure nor the concentration of the riboflavin solution from 0.1% to 1% improved riboflavin penetration through the epithelium. When using riboflavin-5-phosphate nanoemulsion for 240 minutes, we found no difference between the mean absorption coefficients to the standard cross-linking protocol (p = 0.54). Riboflavin nanoemulsion was able to penetrate the corneal epithelium, achieving, after 240 minutes, greater stromal concentration when compared to debrided corneas with the standard protocol (p = 0.002). The riboflavin-5-phosphate nanoemulsion diffused better into the stroma than the riboflavin-base nanoemulsion. © 2013 Bottos et al.
dc.languageeng
dc.relationPLOS ONE
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectadhesive agent
dc.subjectflavine mononucleotide
dc.subjectphosphatidylcholine
dc.subjectpolysorbate 20
dc.subjectanimal tissue
dc.subjectcentral corneal thickness
dc.subjectcontrolled study
dc.subjectcornea epithelium
dc.subjectcornea stroma
dc.subjectdrug absorption
dc.subjectdrug diffusion
dc.subjectdrug penetration
dc.subjectdrug stability
dc.subjecthydrophobicity
dc.subjectlong term exposure
dc.subjectnanoemulsion
dc.subjectnanopharmaceutics
dc.subjectnonhuman
dc.subjectphysical chemistry
dc.subjectprotein cross linking
dc.subjectrabbit
dc.subjectzeta potential
dc.titleCorneal Absorption of a New Riboflavin-Nanostructured System for Transepithelial Collagen Cross-Linking
dc.typeOtro


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