The histidine kinase slnCl1 of Colletotrichum lindemuthianum as a pathogenicity factor against Phaseolus vulgaris L

dc.creatorNogueira, Guilherme Bicalho
dc.creatorSantos, Leandro Vieira dos
dc.creatorQueiroz, Casley Borges de
dc.creatorCorrêa, Thamy Lívia Ribeiro
dc.creatorMenicucci, Renato Pedrozo
dc.creatorBazzolli, Denise Mara Soares
dc.creatorAraújo, Elza Fernandes de
dc.creatorQueiroz, Marisa Vieira de
dc.date2019-03-14T17:28:11Z
dc.date2019-03-14T17:28:11Z
dc.date2019-02
dc.date.accessioned2023-09-27T21:36:04Z
dc.date.available2023-09-27T21:36:04Z
dc.identifier0944-5013
dc.identifierhttps://doi.org/10.1016/j.micres.2018.10.005
dc.identifierhttp://www.locus.ufv.br/handle/123456789/23942
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8963293
dc.descriptionColletotrichum lindemuthianum, the causal agent of anthracnose, is responsible for significant damage in the common bean (Phaseolus vulgaris L.). Unraveling the genetic mechanisms involved in the plant/pathogen interaction is a powerful approach for devising efficient methods to control this disease. In the present study, we employed the Restriction Enzyme-Mediated Integration (REMI) methodology to identify the gene slnCl1, encoding a histidine kinase protein, as involved in pathogenicity. The mutant strain, MutCl1, generated by REMI, showed an insertion in the slnCl1 gene, deficiency of the production and melanization of appressoria, as well as the absence of pathogenicity on bean leaves when compared with the wild-type strain. The slnCl1 gene encodes a histidine kinase class IV called SlnCl1 showing identity of 97% and 83% with histidine kinases from Colletotrichum orbiculare and Colletotrichum gloesporioides, respectively. RNA interference was used for silencing the histidine kinase gene and confirm slnCl1 as a pathogenicity factor. Furthermore, we identified four major genes involved in the RNA interference-mediated gene silencing in Colletotrichum spp. and demonstrated the functionality of this process in C. lindemuthianum. Silencing of the EGFP reporter gene and slnCl1 were demonstrated using qPCR. This work reports for the first time the isolation and characterization of a HK in C. lindemuthianum and the occurrence of gene silencing mediated by RNA interference in this organism, demonstrating its potential use in the functional characterization of pathogenicity genes.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherMicrobiological Research
dc.relationVolume 219, Pages 110-122, February 2019
dc.rightsElsevier B. V.
dc.subjectColletotrichum lindemuthianum
dc.subjectHistidine kinase
dc.subjectTwo-component system
dc.subjectRNA interference
dc.subjectPathogenicity
dc.titleThe histidine kinase slnCl1 of Colletotrichum lindemuthianum as a pathogenicity factor against Phaseolus vulgaris L
dc.typeArtigo


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