Acyl homoserine lactone changes the abundance of proteins and the levels of organic acids associated with stationary phase in Salmonella Enteritidis

dc.creatorAlmeida, Felipe Alvesde
dc.creatorPimentel-Filho, Natan de Jesus
dc.creatorCarrijo, Lanna Clícia
dc.creatorBento, Claudia Braga Pereira
dc.creatorBaracat-Pereira, Maria Cristina
dc.creatorPinto, Uelinton Manoel
dc.creatorOliveira, Leandro Licursi de
dc.creatorVanetti, Maria Cristina Dantas
dc.date2018-04-06T10:06:01Z
dc.date2018-04-06T10:06:01Z
dc.date2016-12-01
dc.date.accessioned2023-09-27T21:33:40Z
dc.date.available2023-09-27T21:33:40Z
dc.identifier08824010
dc.identifierhttps://doi.org/10.1016/j.micpath.2016.11.027
dc.identifierhttp://www.locus.ufv.br/handle/123456789/18668
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8962634
dc.descriptionO artigo não contém resumo em poruguês.
dc.descriptionQuorum sensing (QS) is cell-cell communication mechanism mediated by signaling molecules known as autoinducers (AIs) that lead to differential gene expression. Salmonella is unable to synthesize the AI-1 acyl homoserine lactone (AHL), but is able to recognize AHLs produced by other microorganisms through SdiA protein. Our study aimed to evaluate the influence of AI-1 on the abundance of proteins and the levels of organic acids of Salmonella Enteritidis. The presence of N-dodecyl-homoserine lactone (C12-HSL) did not interfere on the growth or the total amount of extracted proteins of Salmonella. However, the abundance of the proteins PheT, HtpG, PtsI, Adi, TalB, PmgI (or GpmI), Eno, and PykF enhanced while the abundance of the proteins RplB, RplE, RpsB, Tsf, OmpA, OmpC, OmpD, and GapA decreased when Salmonella Enteritidis was anaerobically cultivated in the presence of C12-HSL. Additionally, the bacterium produced less succinic, lactic, and acetic acids in the presence of C12-HSL. However, the concentration of extracellular formic acid reached 20.46 mM after 24 h and was not detected when the growth was in the absence of AI-1. Considering the cultivation period for protein extraction, their abundance, process and function, as well as the levels of organic acids, we observed in cells cultivated in presence of C12-HSL a correlation with what is described in the literature as entry into the stationary phase of growth, mainly related to nitrogen and amino acid starvation and acid stress. Further studies are needed in order to determine the specific role of the differentially abundant proteins and extracellular organic acids secreted by Salmonella in the presence of quorum sensing signaling molecules.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherMicrobial Pathogenesis
dc.relationv. 102, p. 148-159, Janeiro 2017
dc.rightsOpen Access
dc.subjectAutoinducer
dc.subjectDifferentially abundant proteins
dc.subjectFormic acid
dc.subjectStarvation
dc.subjectStationary phase
dc.titleAcyl homoserine lactone changes the abundance of proteins and the levels of organic acids associated with stationary phase in Salmonella Enteritidis
dc.typeArtigo


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