Screening of genomic libraries

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorNovelli, Valdenice M.
dc.creatorCristofani-Yaly, Mariângela
dc.creatorBastianel, Marinês
dc.creatorPalmieri, Dario A.
dc.creatorMachado, Marcos A.
dc.date2014-05-27T11:29:29Z
dc.date2016-10-25T18:48:21Z
dc.date2014-05-27T11:29:29Z
dc.date2016-10-25T18:48:21Z
dc.date2013-05-10
dc.date.accessioned2017-04-06T02:23:42Z
dc.date.available2017-04-06T02:23:42Z
dc.identifierMethods in Molecular Biology, v. 1006, p. 17-24.
dc.identifier1064-3745
dc.identifierhttp://hdl.handle.net/11449/75377
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75377
dc.identifier10.1007/978-1-62703-389-3_2
dc.identifier2-s2.0-84877103470
dc.identifierhttp://dx.doi.org/10.1007/978-1-62703-389-3_2
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896123
dc.descriptionMicrosatellites, or simple sequence repeats (SSRs), have proven to be an important molecular marker in plant genetics and breeding research. The main strategies to obtain these markers can be through genomic DNA and from expressed sequence tags (ESTs) from mRNA/cDNA libraries. Genetic studies using microsatellite markers have increased rapidly because they can be highly polymorphic, codominant markers and they show heterozygous conserved sequences. Here, we describe a methodology to obtain microsatellite using the enrichment library of DNA genomic sequences. This method is highly efficient to development microsatellite markers especially in plants that do not have available ESTs or genome databases. This methodology has been used to enrich SSR marker libraries in Citrus spp., an important tool to genotype germplasm, to select zygotic hybrids, and to saturate genetic maps in breeding programs. © Springer Science+Business Media, LLC 2013.
dc.languageeng
dc.relationMethods in Molecular Biology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEnrichment methods
dc.subjectMicrosatellites
dc.subjectMolecular markers
dc.subjectgenomic DNA
dc.subjectCitrus
dc.subjectcodominance
dc.subjectDNA library
dc.subjectexpressed sequence tag
dc.subjectgene library
dc.subjectgene mapping
dc.subjectgene sequence
dc.subjectgenetic screening
dc.subjectgenotype
dc.subjectgermplasm
dc.subjectheterozygote
dc.subjecthybrid
dc.subjectmicrosatellite marker
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectplant breeding
dc.subjectpriority journal
dc.subjectsimple sequence repeat
dc.subjectBreeding
dc.subjectComputational Biology
dc.subjectDNA, Plant
dc.subjectGenetic Markers
dc.subjectGenome, Plant
dc.subjectGenomic Library
dc.subjectMicrosatellite Repeats
dc.titleScreening of genomic libraries
dc.typeOtro


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