Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorLima, Fabio Mitsuo
dc.creatorSouza, Renata Torres
dc.creatorSantori, Fábio Rinaldo
dc.creatorSantos, Michele Fernandes
dc.creatorCortez, Danielle Rodrigues
dc.creatorBarros, Roberto Moraes
dc.creatorCano, Maria Isabel
dc.creatorValadares, Helder Magno Silva
dc.creatorMacedo, Andréa Mara
dc.creatorMortara, Renato Arruda
dc.creatorda Silveira, José Franco
dc.date2014-05-27T11:29:28Z
dc.date2016-10-25T18:48:19Z
dc.date2014-05-27T11:29:28Z
dc.date2016-10-25T18:48:19Z
dc.date2013-05-07
dc.date.accessioned2017-04-06T02:23:35Z
dc.date.available2017-04-06T02:23:35Z
dc.identifierPLoS ONE, v. 8, n. 5, 2013.
dc.identifier1932-6203
dc.identifierhttp://hdl.handle.net/11449/75363
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75363
dc.identifier10.1371/journal.pone.0063738
dc.identifierWOS:000319654700183
dc.identifier2-s2.0-84877140924.pdf
dc.identifier2-s2.0-84877140924
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0063738
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896109
dc.descriptionTrypanosoma cruzi comprises a pool of populations which are genetically diverse in terms of DNA content, growth and infectivity. Inter- and intra-strain karyotype heterogeneities have been reported, suggesting that chromosomal rearrangements occurred during the evolution of this parasite. Clone D11 is a single-cell-derived clone of the T. cruzi G strain selected by the minimal dilution method and by infecting Vero cells with metacyclic trypomastigotes. Here we report that the karyotype of clone D11 differs from that of the G strain in both number and size of chromosomal bands. Large chromosomal rearrangement was observed in the chromosomes carrying the tubulin loci. However, most of the chromosome length polymorphisms were of small amplitude, and the absence of one band in clone D11 in relation to its reference position in the G strain could be correlated to the presence of a novel band migrating above or below this position. Despite the presence of chromosomal polymorphism, large syntenic groups were conserved between the isolates. The appearance of new chromosomal bands in clone D11 could be explained by chromosome fusion followed by a chromosome break or interchromosomal exchange of large DNA segments. Our results also suggest that telomeric regions are involved in this process. The variant represented by clone D11 could have been induced by the stress of the cloning procedure or could, as has been suggested for Leishmania infantum, have emerged from a multiclonal, mosaic parasite population submitted to frequent DNA amplification/deletion events, leading to a 'mosaic' structure with different individuals having differently sized versions of the same chromosomes. If this is the case, the variant represented by clone D11 would be better adapted to survive the stress induced by cloning, which includes intracellular development in the mammalian cell. Karyotype polymorphism could be part of the T. cruzi arsenal for responding to environmental pressure. © 2013 Lima et al.
dc.languageeng
dc.relationPLOS ONE
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectalpha tubulin
dc.subjectbeta tubulin
dc.subjectalpha tubulin gene
dc.subjectbeta tubulin gene
dc.subjectchromosome band
dc.subjectchromosome breakage
dc.subjectchromosome number
dc.subjectchromosome polymorphism
dc.subjectchromosome rearrangement
dc.subjectchromosome size
dc.subjectclonal variation
dc.subjectcontrolled study
dc.subjectDNA sequence
dc.subjectepimastigote
dc.subjectgene amplification
dc.subjectgene cluster
dc.subjectgene deletion
dc.subjectgene dosage
dc.subjectgene fusion
dc.subjectgene locus
dc.subjectgene translocation
dc.subjectgenetic variability
dc.subjectgenome analysis
dc.subjectkaryotyping
dc.subjectmolecular cloning
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectparasite isolation
dc.subjectspecies difference
dc.subjecttandem repeat
dc.subjecttelomere
dc.subjecttelomere homeostasis
dc.subjectTrypanosoma cruzi
dc.titleInterclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain
dc.typeOtro


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