Effects of zoledronic acid on odontoblast-like cells

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBasso, F. G.
dc.creatorTurrioni, A. P S
dc.creatorHebling, J.
dc.creatorDe Souza Costa, C. A.
dc.date2014-05-27T11:29:01Z
dc.date2016-10-25T18:47:53Z
dc.date2014-05-27T11:29:01Z
dc.date2016-10-25T18:47:53Z
dc.date2013-05-01
dc.date.accessioned2017-04-06T02:21:54Z
dc.date.available2017-04-06T02:21:54Z
dc.identifierArchives of Oral Biology, v. 58, n. 5, p. 467-473, 2013.
dc.identifier0003-9969
dc.identifierhttp://hdl.handle.net/11449/75203
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75203
dc.identifier10.1016/j.archoralbio.2012.09.016
dc.identifierWOS:000318322000004
dc.identifier2-s2.0-84876063550.pdf
dc.identifier2-s2.0-84876063550
dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2012.09.016
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895950
dc.descriptionThe aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5 × 104 cells/cm2) were seeded for 48 h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24 h, ZOL (1 or 5 μM) was added to the medium and maintained in contact with the cells for 24 h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability-MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five μM ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1 μM ZOL caused a significant increase in Col-I expression. Although 5 μM ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p < 0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex. © 2012 Elsevier Ltd.
dc.languageeng
dc.relationArchives of Oral Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBisphosphonates
dc.subjectCell culture
dc.subjectCytotoxicity
dc.subjectalkaline phosphatase
dc.subjectbisphosphonic acid derivative
dc.subjectbone density conservation agent
dc.subjectcollagen type 1
dc.subjectdentin sialophosphoprotein
dc.subjectimidazole derivative
dc.subjectphosphoprotein
dc.subjectprotein synthesis inhibitor
dc.subjectscleroprotein
dc.subjectsialoglycoprotein
dc.subjectsuccinate dehydrogenase
dc.subjectzoledronic acid
dc.subjectbiosynthesis
dc.subjectcell culture
dc.subjectcell survival
dc.subjectdose response
dc.subjectdrug antagonism
dc.subjectdrug effect
dc.subjectgene expression regulation
dc.subjecthuman
dc.subjectodontoblast
dc.subjectAlkaline Phosphatase
dc.subjectBone Density Conservation Agents
dc.subjectCell Survival
dc.subjectCells, Cultured
dc.subjectCollagen Type I
dc.subjectDiphosphonates
dc.subjectDose-Response Relationship, Drug
dc.subjectExtracellular Matrix Proteins
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectHumans
dc.subjectImidazoles
dc.subjectOdontoblasts
dc.subjectPhosphoproteins
dc.subjectProtein Synthesis Inhibitors
dc.subjectSialoglycoproteins
dc.subjectSuccinate Dehydrogenase
dc.subjectBovinae
dc.titleEffects of zoledronic acid on odontoblast-like cells
dc.typeOtro


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