In vitro organogenesis from root culture segments of Bixa orellana L. (Bixaceae)

dc.creatorCruz, Ana Claudia Ferreira da
dc.creatorRocha, Diego Ismael
dc.creatorIarema, Lourdes
dc.creatorVentrella, Marília Contim
dc.creatorOtoni, Wagner Campos
dc.creatorCosta, Marcio Gilberto Cardoso
dc.creatorPaiva Neto, Vespasiano Borges
dc.date2019-02-06T15:48:10Z
dc.date2019-02-06T15:48:10Z
dc.date2014-03
dc.date.accessioned2023-09-27T21:21:30Z
dc.date.available2023-09-27T21:21:30Z
dc.identifier1475-2689
dc.identifierhttps://doi.org/10.1007/s11627-013-9580-2
dc.identifierhttp://www.locus.ufv.br/handle/123456789/23360
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8959264
dc.descriptionThe organogenic potential of root explants derived from cultured seedlings of Bixa orellana L. (annatto) was investigated in response to different incubation conditions and either 4.44 μM 6-benzyladenine, 4.54 μM Thidiazuron, or 4.56 μM Zeatin. Explants cultured in liquid media with agitation generally showed better development of adventitious buds versus explants cultured on semi-solid media. The most adventitious buds developed from explants cultured in liquid media under a 16-h photoperiod. Use of Zeatin and Thidiazuron promoted the development of more adventitious buds than 6-benzyladenine but morphological abnormalities among regenerating shoots and plants were observed. Fewer adventitious buds developed from explants cultured in liquid media supplemented with 6-benzyladenine, but the buds gave rise to the highest percentage of morphologically normal regenerated plants. Histological analyses showed that adventitious buds originated from cell proliferation within the pericycle, opposite the protoxylem poles of the explant. Seedling root tissue is useful for in vitro propagation of B. orellana.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherIn Vitro Cellular & Developmental Biology - Plant
dc.relationVolume 50, Issue 1, Pages 76–83, March 2014
dc.rightsThe Society for In Vitro Biology
dc.subjectAnnatto
dc.subjectAdventitious bud development
dc.subjectCytokinins In vitro plant regeneration
dc.subjectLiquid cultures
dc.subjectRoot explants
dc.titleIn vitro organogenesis from root culture segments of Bixa orellana L. (Bixaceae)
dc.typeArtigo


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