Bovine herpesvirus-5 infection in a rabbit experimental model: Immunohistochemical study of the cellular response in the CNS

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMachado, Gisele Fabrino
dc.creatorBernardi, Fernanda
dc.creatorHosomi, Fernando Y.M.
dc.creatorPeiró, Juliana Regina
dc.creatorWeiblen, Rudi
dc.creatorRoehe, Paulo M.
dc.creatorAlessi, Antonio C.
dc.creatorMelo, Guilherme D.
dc.creatorRamos, Adriano T.
dc.creatorMaiorka, Paulo C.
dc.date2014-05-27T11:28:45Z
dc.date2016-10-25T18:46:02Z
dc.date2014-05-27T11:28:45Z
dc.date2016-10-25T18:46:02Z
dc.date2013-04-01
dc.date.accessioned2017-04-06T02:18:11Z
dc.date.available2017-04-06T02:18:11Z
dc.identifierMicrobial Pathogenesis, v. 57, p. 10-16.
dc.identifier0882-4010
dc.identifier1096-1208
dc.identifierhttp://hdl.handle.net/11449/74935
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74935
dc.identifier10.1016/j.micpath.2013.01.003
dc.identifierWOS:000316921400002
dc.identifier2-s2.0-84873968605.pdf
dc.identifier2-s2.0-84873968605
dc.identifierhttp://dx.doi.org/10.1016/j.micpath.2013.01.003
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895694
dc.descriptionSince little information is available regarding cellular antigen mapping and the involvement of non-neuronal cells in the pathogenesis of bovine herpesvirus type 5 (BHV-5) infection, it were determined the BHV-5 distribution, the astrocytic reactivity, the involvement of lymphocytes and the presence of matrix metalloproteinase (MMP)-9 in the brain of rabbits experimentally infected with BHV-5. Twelve New Zealand rabbits that were seronegative for BHV-5 were used for virus inoculation, and five rabbits were used as mock-infected controls. The rabbits were kept in separate areas and were inoculated intranasally with 500 μl of virus suspension (EVI 88 Brazilian isolate) into each nostril (virus titer, 107.5 TCID50). Control rabbits were inoculated with the same volume of minimum essential medium. Five days before virus inoculation, the rabbits were submitted to daily administration of dexamethasone. After virus inoculation, the rabbits were monitored clinically on a daily basis. Seven rabbits showed respiratory symptoms and four animals exhibited neurological symptoms. Tissue sections were collected for histological examination and immunohistochemistry to examine BHV-5 antigens, astrocytes, T and B lymphocytes and MMP-9. By means of immunohistochemical and PCR methods, BHV-5 was detected in the entire brain of the animals which presented with neurological symptoms, especially in the trigeminal ganglion and cerebral cortices. Furthermore, BHV-5 antigens were detected in neurons and/or other non-neural cells. In addition to the neurons, most infiltrating CD3 T lymphocytes observed in these areas were positive for MMP-9 and also for BHV-5 antigen. These infected cells might contribute to the spread of the virus to the rabbit brain along the trigeminal ganglia and olfactory nerve pathways. © 2013 Elsevier Ltd.
dc.languageeng
dc.relationMicrobial Pathogenesis
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnimal model
dc.subjectAstrocytes
dc.subjectInflammation
dc.subjectLymphocytes
dc.subjectMatrix metalloproteinases
dc.subjectBovine herpespirus 5 antigen
dc.subjectcell antigen
dc.subjectdexamethasone
dc.subjectgelatinase B
dc.subjectunclassified drug
dc.subjectvirus antigen
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectantibody titer
dc.subjectastrocyte
dc.subjectB lymphocyte
dc.subjectBovine herpes virus
dc.subjectbrain
dc.subjectbrain cortex
dc.subjectCD3+ T lymphocyte
dc.subjectcellular immunity
dc.subjectcentral nervous system
dc.subjectcontrolled study
dc.subjectenzyme activity
dc.subjectexperimental model
dc.subjectherpes virus infection
dc.subjectimmunohistochemistry
dc.subjectimmunopathogenesis
dc.subjectinoculation
dc.subjectlymphocyte
dc.subjectlymphocytic infiltration
dc.subjectmale
dc.subjectnerve cell
dc.subjectneurologic disease
dc.subjectnonhuman
dc.subjectnose
dc.subjectpriority journal
dc.subjectrabbit
dc.subjectrespiratory tract disease
dc.subjectsuspension cell culture
dc.subjectsymptomatology
dc.subjectT lymphocyte
dc.subjecttrigeminus ganglion
dc.subjectAnimals
dc.subjectBrain
dc.subjectCattle
dc.subjectDisease Models, Animal
dc.subjectEncephalitis, Viral
dc.subjectHerpesviridae Infections
dc.subjectHerpesvirus 5, Bovine
dc.subjectMale
dc.subjectMatrix Metalloproteinase 9
dc.subjectMeningoencephalitis
dc.subjectRabbits
dc.titleBovine herpesvirus-5 infection in a rabbit experimental model: Immunohistochemical study of the cellular response in the CNS
dc.typeOtro


Este ítem pertenece a la siguiente institución