Development and validation of the high performance liquid chromatography–ion exclusion method for detection of lactic acid in milk

dc.creatorMilagres, Maria Patricia
dc.creatorBrandão, Sebastião César Cardoso
dc.creatorMinim, Valéria Paula Rodrigues
dc.creatorMinim, Luis Antonio
dc.creatorMagalhães, Mirella Araujo
dc.date2017-11-16T16:04:05Z
dc.date2017-11-16T16:04:05Z
dc.date2012-05-18
dc.date.accessioned2023-09-27T21:13:06Z
dc.date.available2023-09-27T21:13:06Z
dc.identifier0308-8146
dc.identifierhttps://doi.org/10.1016/j.foodchem.2012.05.047
dc.identifierhttp://www.locus.ufv.br/handle/123456789/13132
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8956743
dc.descriptionThe present work reports the development and validation of an analytical method for lactic acid quantification in milk by ion exclusion–HPLC with UV detection. This method showed good precision (variation coefficient <7.0%) and good accuracy (97.6–99.6%). Calibration curves were linear (R2 > 0.996) at the tested range of 5.0–25.0 mmol L−1; and the detection and quantification limits were 0.5 and 1.0 mmol L−1, respectively. Analysis of lactic acid in fermented milk (FM) or NaOH neutralised fermented milk (FNM) was performed using samples obtained during 9 h of fermentation. An ANOVA analysis combined with the Tukey test was used to show that the method was insensitive to the differences between FM and FNM. The proposed method therefore proved to be a useful quality control tool in laboratories because it was able to determine milk acidity even in adulterated samples.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherFood Chemistry
dc.relationVolume 135, Issue 3, Pages 1078-1082, December 2012
dc.rightsOpen Access
dc.subjectMethod development
dc.subjectMethod validation
dc.subjectIon exclusion
dc.subjectHigh-performance
dc.subjectliquid chromatography
dc.subjectLactic acid
dc.subjectMilk
dc.titleDevelopment and validation of the high performance liquid chromatography–ion exclusion method for detection of lactic acid in milk
dc.typeArtigo


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