Polymerase chain reaction assay based on ratA gene allows differentiation between Salmonella enterica subsp. enterica serovar Gallinarum biovars Gallinarum and Pullorum

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBatista, Diego Felipe Alves
dc.creatorde Freitas Neto, Oliveiro Caetano
dc.creatorLopes, Priscila Diniz
dc.creatorde Almeida, Adriana Maria
dc.creatorBarrow Jr., Paul Andrew
dc.creatorBerchieri, Angelo
dc.date2014-05-27T11:28:44Z
dc.date2016-10-25T18:45:55Z
dc.date2014-05-27T11:28:44Z
dc.date2016-10-25T18:45:55Z
dc.date2013-03-28
dc.date.accessioned2017-04-06T02:17:42Z
dc.date.available2017-04-06T02:17:42Z
dc.identifierJournal of Veterinary Diagnostic Investigation, v. 25, n. 2, p. 259-262, 2013.
dc.identifier1040-6387
dc.identifier1943-4936
dc.identifierhttp://hdl.handle.net/11449/74889
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74889
dc.identifier10.1177/1040638713479361
dc.identifierWOS:000319220300013
dc.identifier2-s2.0-84875309804.pdf
dc.identifier2-s2.0-84875309804
dc.identifierhttp://dx.doi.org/10.1177/1040638713479361
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895648
dc.descriptionSalmonella Pullorum and Salmonella Gallinarum are classified as biovars of Salmonella enterica subsp. enterica serovar Gallinarum. These salmonellae are the causative agents of Pullorum disease and fowl typhoid, respectively, and are widely distributed throughout the world. Although many developed countries have eradicated these diseases from commercial poultry, they are still the cause of significant economic loss in developing countries. When serovar Gallinarum is isolated, it is difficult to immediately differentiate between biovars because they are antigenically identical by serotyping. However, they cause distinct diseases with different epidemiology, and therefore it is important to differentiate them. This may be done biochemically but takes 2 to 3 days. In the present study, S. Pullorum and S. Gallinarum whole genomes were compared, and 1 genomic region of difference, which is part of the ratA gene, was chosen as a molecular marker for a polymerase chain reaction assay to differentiate rapidly between these organisms. In all, 26 strains of S. Gallinarum and 17 S. Pullorum strains were tested and successfully differentiated by the assay. © 2013 The Author(s).
dc.languageeng
dc.relationJournal of Veterinary Diagnostic Investigation
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGenome
dc.subjectpolymerase chain reaction
dc.subjectrapid differentiation
dc.subjectratA gene
dc.subjectSalmonella Gallinarum
dc.subjectSalmonella Pullorum
dc.subjectbacterial protein
dc.subjectanimal
dc.subjectanimal disease
dc.subjectanimal salmonellosis
dc.subjectbacterial genome
dc.subjectbird disease
dc.subjectclassification
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectmicrobiology
dc.subjectphysiology
dc.subjectpoultry
dc.subjectSalmonella enterica
dc.subjectAnimals
dc.subjectBacterial Proteins
dc.subjectGene Expression Regulation, Bacterial
dc.subjectGenome, Bacterial
dc.subjectPolymerase Chain Reaction
dc.subjectPoultry
dc.subjectPoultry Diseases
dc.subjectSalmonella Infections, Animal
dc.titlePolymerase chain reaction assay based on ratA gene allows differentiation between Salmonella enterica subsp. enterica serovar Gallinarum biovars Gallinarum and Pullorum
dc.typeOtro


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