Differences in skeletal muscle proteolysis in Nellore and Angus cattle might be driven by Calpastatin activity and not the abundance of Calpain/Calpastatin

dc.creatorMartins, T. S.
dc.creatorSanglard, L. M. P.
dc.creatorSilva, W.
dc.creatorChizzotti, M. L.
dc.creatorLadeira, M. M.
dc.creatorSerão, N. V. L.
dc.creatorPaulino, P. V. R.
dc.creatorDuarte, M. S.
dc.date2019-01-11T13:36:01Z
dc.date2019-01-11T13:36:01Z
dc.date2017-12
dc.date.accessioned2023-09-27T21:08:12Z
dc.date.available2023-09-27T21:08:12Z
dc.identifier1469-5146
dc.identifierhttp://dx.doi.org/10.1017/S0021859617000715
dc.identifierhttp://www.locus.ufv.br/handle/123456789/22977
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8955265
dc.descriptionThe present study aimed to explore the molecular factors underlying differences in Calpain/Calpastatin proteolytic system in Nellore and Angus cattle. Longissimus muscle samples were collected in Nellore (n = 6; body weight (BW) = 373 ± 37·3 kg) and Angus (n = 6; BW = 383 ± 23·9 kg) cattle at slaughter for analysis of gene and protein expression, and Calpastatin enzyme activity. Additionally, the myofibrillar fragmentation index was used to quantify the extension of proteolysis in longissimus muscle samples. A greater myofibrillar fragmentation was observed in skeletal muscle of Angus compared with Nellore cattle. Conversely, no differences were found between breeds for mRNA expression of Calpain 1 (CAPN1) and Calpastatin (CAST). Similarly, no differences were observed for the abundance of Calpain and Calpastatin proteins between skeletal muscles of Nellore and Angus cattle. Despite the lack of differences in mRNA and protein abundance, a greater activity of Calpastatin was observed in skeletal muscle of Nellore compared with Angus cattle. These data indicate that the greater proteolysis in skeletal muscle of Angus compared with Nellore cattle is mainly driven by a greater Calpastatin activity rather than Calpain or Calpastatin mRNA and protein expression.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherThe Journal of Agricultural Science
dc.relationVolume 155, Issue 10, Pages 1669- 1676, December 2017
dc.rightsOpen Access
dc.subjectCalpastatin
dc.subjectNellore
dc.subjectAngus
dc.subjectSkeletal muscle proteolysis
dc.titleDifferences in skeletal muscle proteolysis in Nellore and Angus cattle might be driven by Calpastatin activity and not the abundance of Calpain/Calpastatin
dc.typeArtigo


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