A novel transcription factor, ERD15 (Early Responsive to Dehydration 15), connects Endoplasmic Reticulum stress with an osmotic stress-induced cell death signal

dc.creatorAlves, Murilo S.
dc.creatorReis, Pedro A. B.
dc.creatorDadalto, Silvana P.
dc.creatorFaria, Jerusa A. Q. A.
dc.creatorFontes, Elizabeth P. B.
dc.creatorFietto, Luciano G.
dc.date2017-10-09T12:51:23Z
dc.date2017-10-09T12:51:23Z
dc.date2011-04-11
dc.date.accessioned2023-09-27T21:07:58Z
dc.date.available2023-09-27T21:07:58Z
dc.identifier1083-351X
dc.identifierhttp://dx.doi.org/10.1074/jbc.M111.233494
dc.identifierhttp://www.locus.ufv.br/handle/123456789/11940
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8955192
dc.descriptionAs in all other eukaryotic organisms, endoplasmic reticulum (ER) stress triggers the evolutionarily conserved unfolded protein response in soybean, but it also communicates with other adaptive signaling responses, such as osmotic stress-induced and ER stress-induced programmed cell death. These two signaling pathways converge at the level of gene transcription to activate an integrated cascade that is mediated by N-rich proteins (NRPs). Here, we describe a novel transcription factor, GmERD15 (Glycine max Early Responsive to Dehydration 15), which is induced by ER stress and osmotic stress to activate the expression of NRP genes. GmERD15 was isolated because of its capacity to stably associate with the NRP-B promoter in yeast. It specifically binds to a 187-bp fragment of the NRP-B promoter in vitro and activates the transcription of a reporter gene in yeast. Furthermore, GmERD15 was found in both the cytoplasm and the nucleus, and a ChIP assay revealed that it binds to the NRP-B promoter in vivo. Expression of GmERD15 in soybean protoplasts activated the NRP-B promoter and induced expression of the NRP-B gene. Collectively, these results support the interpretation that GmERD15 functions as an upstream component of stress-induced NRP-B-mediated signaling to connect stress in the ER to an osmotic stress-induced cell death signal.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherThe Journal of Biological Chemistry
dc.relationVol. 286, N. 22, pp. 20020 –20030, June 2011
dc.rightsOpen Access
dc.subjectPlant
dc.subjectPlant biology
dc.subjectTranscription factors
dc.subjectIntegration of signaling pathways
dc.subjectER stress
dc.subjectAbiotic stresses
dc.subjectTranscription factors
dc.subjectOxidative stress
dc.subjectER stress
dc.subjectCell death
dc.titleA novel transcription factor, ERD15 (Early Responsive to Dehydration 15), connects Endoplasmic Reticulum stress with an osmotic stress-induced cell death signal
dc.typeArtigo


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