Effect of fluoride-treated enamel on indirect cytotoxicity of a16% carbamide peroxide bleaching gel to pulp cells

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSoares, Diana Gabriela
dc.creatorRibeiro, Ana Paula Dias
dc.creatorLima, Adriano Fonseca
dc.creatorSacono, Nancy Tomoko
dc.creatorHebling, Josimeri
dc.creatorCosta, Carlos Alberto de Souza
dc.date2014-05-27T11:28:36Z
dc.date2016-10-25T18:45:07Z
dc.date2014-05-27T11:28:36Z
dc.date2016-10-25T18:45:07Z
dc.date2013-03-01
dc.date.accessioned2017-04-06T02:15:43Z
dc.date.available2017-04-06T02:15:43Z
dc.identifierBrazilian Dental Journal, v. 24, n. 2, p. 121-127, 2013.
dc.identifier0103-6440
dc.identifier1806-4760
dc.identifierhttp://hdl.handle.net/11449/74737
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74737
dc.identifier10.1590/0103-6440201302161
dc.identifierS0103-6440201302161
dc.identifier2-s2.0-84878463744.pdf
dc.identifier2-s2.0-84878463744
dc.identifierhttp://dx.doi.org/10.1590/0103-6440201302161
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895497
dc.descriptionThe aim of this study was to evaluate the possibility of fluoride solutions applied to enamel to protect pulp cells against the trans-enamel and transdentinal cytotoxicity of a 16% carbamide peroxide (CP) bleaching gel. The CP gel was applied to enamel/ dentin discs adapted to artificial pulp chambers (8 h/day) during 1, 7 or 14 days, followed by fluoride (0.05% or 0.2%) application for 1 min. The extracts (culture medium in contact with dentin) were applied to MDPC-23 cells for 1 h, and cell metabolism (MTT assay), alkaline phosphatase (ALP) activity and cell membrane damage (flow cytometry) were analyzed. Knoop microhardness of enamel was also evaluated. Data were analyzed statistically by ANOVA and Kruskal-Wallis tests (a=0.05). For the MTT assay and ALP activity, significant reductions between the control and the bleached groups were observed (p<0.05). No statistically significant difference occurred among bleached groups (p>0.05), regardless of fluoride application or treatment days. Flow cytometry analysis demonstrated 30% of cell membrane damage in all bleached groups. After 14 days of treatment, the fluoride-treated enamel presented significantly higher microhardness values than the bleached-only group (p<0.05). It was concluded that, regardless of the increase in enamel hardness due to the application of fluoride solutions, the treated enamel surface did not prevent the toxic effects caused by the 16% CP gel to odontoblast-like cells.
dc.languageeng
dc.relationBrazilian Dental Journal
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCarbamide peroxide
dc.subjectCytotoxicity
dc.subjectOdontoblast
dc.subjectTooth bleaching
dc.titleEffect of fluoride-treated enamel on indirect cytotoxicity of a16% carbamide peroxide bleaching gel to pulp cells
dc.typeOtro


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