Reduced in vitro immune response on titania nanotube arrays compared to titanium surface

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSmith, Barbara S.
dc.creatorCapellato, Patricia
dc.creatorKelley, Sean
dc.creatorGonzalez-Juarrero, Mercedes
dc.creatorPopat, Ketul C.
dc.date2014-05-27T11:28:35Z
dc.date2016-10-25T18:45:01Z
dc.date2014-05-27T11:28:35Z
dc.date2016-10-25T18:45:01Z
dc.date2013-03-01
dc.date.accessioned2017-04-06T02:15:22Z
dc.date.available2017-04-06T02:15:22Z
dc.identifierBiomaterials Science, v. 1, n. 3, p. 322-332, 2013.
dc.identifier2047-4830
dc.identifier2047-4849
dc.identifierhttp://hdl.handle.net/11449/74698
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74698
dc.identifier10.1039/c2bm00079b
dc.identifierWOS:000330133600010
dc.identifier2-s2.0-84885332118
dc.identifierhttp://dx.doi.org/10.1039/c2bm00079b
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895458
dc.descriptionMaterial surfaces that provide biomimetic cues, such as nanoscale architectures, have been shown to alter cell/biomaterial interactions. Recent studies have identified titania nanotube arrays as strong candidates for use in interfaces on implantable devices due to their ability to elicit improved cellular functionality. However, limited information exists regarding the immune response of nanotube arrays. Thus, in this study, we have investigated the short- and long-term immune cell reaction of titania nanotube arrays. Whole blood lysate (containing leukocytes, thrombocytes and trace amounts of erythrocytes), isolated from human blood, were cultured on titania nanotube arrays and biomedical grade titanium (as a control) for 2 hours and 2 and 7 days. In order to determine the in vitro immune response on titania nanotube arrays, immune cell functionality was evaluated by cellular viability, adhesion, proliferation, morphology, cytokine/chemokine expression, with and without lipopolysaccharide (LPS), and nitric oxide release. The results presented in this study indicate a decrease in short- and long-term monocyte, macrophage and neutrophil functionality on titania nanotube arrays as compared to the control substrate. This work shows a reduced stimulation of the immune response on titania nanotube arrays, identifying this specific nanoarchitecture as a potentially optimal interface for implantable biomedical devices. © 2013 The Royal Society of Chemistry.
dc.languageeng
dc.relationBiomaterials Science
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCellular viability
dc.subjectImplantable biomedical devices
dc.subjectImplantable devices
dc.subjectLimited information
dc.subjectLipopolysaccharides
dc.subjectNanoscale architectures
dc.subjectNitric oxide release
dc.subjectTitania nanotube arrays
dc.subjectBiomimetics
dc.subjectBlood
dc.subjectImmune system
dc.subjectImplants (surgical)
dc.subjectNanotubes
dc.subjectNitric oxide
dc.subjectTitanium dioxide
dc.subjectCellular arrays
dc.titleReduced in vitro immune response on titania nanotube arrays compared to titanium surface
dc.typeOtro


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