dc.creator | Costa, Maximiller D. L. | |
dc.creator | Reis, Pedro A. B. | |
dc.creator | Valente, Maria Anete S. | |
dc.creator | Irsigler, André S. T. | |
dc.creator | Carvalho, Claudine M. | |
dc.creator | Loureiro, Marcelo E. | |
dc.creator | Aragão, Francisco J. L. | |
dc.creator | Boston, Rebecca S. | |
dc.creator | Fietto, Luciano G. | |
dc.creator | Fontes, Elizabeth P. B. | |
dc.date | 2018-04-23T17:37:03Z | |
dc.date | 2018-04-23T17:37:03Z | |
dc.date | 2008-05-19 | |
dc.date.accessioned | 2023-09-27T21:05:50Z | |
dc.date.available | 2023-09-27T21:05:50Z | |
dc.identifier | 1083351X | |
dc.identifier | https://doi.org/10.1074/jbc.M802654200 | |
dc.identifier | http://www.locus.ufv.br/handle/123456789/19047 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8954493 | |
dc.description | NRPs (N-rich proteins) were identified as targets of a novel
adaptive pathway that integrates endoplasmic reticulum (ER)
and osmotic stress signals based on coordinate regulation and
synergistic up-regulation by tunicamycin and polyethylene gly-
col treatments. This integrated pathway diverges from the
molecular chaperone-inducing branch of the unfolded protein
response (UPR) in several ways. While UPR-specific targets
were inversely regulated by ER and osmotic stresses, NRPs
required both signals for full activation. Furthermore, BiP
(binding protein) overexpression in soybean prevented activa-
tion of the UPR by ER stress inducers, but did not affect activa-
tion of NRPs. We also found that this integrated pathway trans-
duces a PCD signal generated by ER and osmotic stresses that
result in the appearance of markers associated with leaf senes-
cence. Overexpression of NRPs in soybean protoplasts induced
caspase-3-like activity and promoted extensive DNA fragmen-
tation. Furthermore, transient expression of NRPs in planta
caused leaf yellowing, chlorophyll loss, malondialdehyde pro-
duction, ethylene evolution, and induction of the senescence
marker gene CP1. This phenotype was alleviated by the cytoki-
nin zeatin, a potent senescence inhibitor. Collectively, these
results indicate that ER stress induces leaf senescence through
activation of plant-specific NRPs via a novel branch of the ER
stress response. | |
dc.format | pdf | |
dc.format | application/pdf | |
dc.language | eng | |
dc.publisher | The Journal of biological chemistry | |
dc.relation | v. 283, n. 29, p. 20209 –20219, July 2008 | |
dc.rights | American Society for Biochemistry and Molecular Biology, Inc. | |
dc.subject | Endoplasmic reticulum | |
dc.subject | Asparagine-rich | |
dc.title | A New branch of endoplasmic reticulum stress signaling and the osmotic signal converge on Plant-specific Asparagine-rich proteins to promote cell death | |
dc.type | Artigo | |