Controlling cooperativity in β-cyclodextrin–DNA binding reactions

dc.creatorRocha, M. S.
dc.creatorAlves, P. S.
dc.creatorMesquita, O. N.
dc.date2018-05-04T15:26:31Z
dc.date2018-05-04T15:26:31Z
dc.date2015-08-26
dc.date.accessioned2023-09-27T21:05:41Z
dc.date.available2023-09-27T21:05:41Z
dc.identifier19487185
dc.identifierhttp://dx.doi.org/10.1021/acs.jpclett.5b01603
dc.identifierhttp://www.locus.ufv.br/handle/123456789/19339
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8954432
dc.descriptionWe have investigated the interaction between the native neutral β- cyclodextrin (CD) and the DNA molecule by performing single-molecule stretching experiments with optical tweezers. In particular, we have monitored the changes of the mechanical properties of the CD−DNA complexes as a function of the CD concentration in the sample. By using a quenched disorder statistical model, we were also capable to extract important physicochemical information (equilibrium binding constants, cooperativity degree) of such interaction from the mechanical data. In addition, we have found that the interaction occurs by two different mechanisms, first with the formation of relatively large CD clusters along the double helix, which thereafter can locally denature the DNA molecule by forming hydrogen bonds with the base pairs that eventually flip out. A prediction of our quenched disorder model was that cooperativity could be controlled by adjusting the surface charge of β-CD molecules. This prediction is confirmed in the present work.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherThe Journal of Physical Chemistry Letters
dc.relationv. 6, n. 18, p. 3549–3554, aug. 2015
dc.rightsAmerican Chemical Society
dc.subjectCooperative binding
dc.subjectCyclodextrin
dc.subjectDNA
dc.subjectPersistence length
dc.titleControlling cooperativity in β-cyclodextrin–DNA binding reactions
dc.typeArtigo


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