| dc.creator | Lopes, K.V.G. | |
| dc.creator | Silva, L.B. | |
| dc.creator | Reis, A.P. | |
| dc.creator | Oliveira, M.G.A. | |
| dc.creator | Guedes, R.N.C. | |
| dc.date | 2018-05-09T11:10:56Z | |
| dc.date | 2018-05-09T11:10:56Z | |
| dc.date | 2010-03-17 | |
| dc.date.accessioned | 2023-09-27T21:04:43Z | |
| dc.date.available | 2023-09-27T21:04:43Z | |
| dc.identifier | 0022-1910 | |
| dc.identifier | https://doi.org/10.1016/j.jinsphys.2010.02.020 | |
| dc.identifier | http://www.locus.ufv.br/handle/123456789/19401 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8954106 | |
| dc.description | Fitness cost is usually associated with insecticide resistance and may be mitigated by increased energy accumulation and mobilization. Preliminary evidence in the maize weevil (Coleoptera: Curculionidae) suggested possible involvement of amylases in such phenomenon. Therefore, α-amylases were purified from an insecticide-susceptible and two insecticide-resistant strains (one with fitness cost [resistant cost strain], and the other without it [resistant no-cost strain]). The main α-amylase of each strain was purified by glycogen precipitation and ion-exchange chromatography (≥70-fold purification, ≤19% yield). Single α-amylase bands with the same molecular mass (53.7 kDa) were revealed for each insect strain. Higher activity was obtained at 35–40 °C and at pH 5.0–7.0 for all of the strains. The α-amylase from the resistant no-cost strain exhibited higher activity towards starch and lower inhibition by acarbose and wheat amylase inhibitors. Opposite results were observed for the α-amylase from the resistant cost strain. Although the α-amylase from the resistant cost strain exhibited higher affinity to starch (i.e., lower Km), its Vmax-value was the lowest among the strains, particularly the resistant no-cost strain. Such results provide support for the hypothesis that enhanced α-amylase activity may be playing a major role in mitigating fitness costs associated with insecticide resistance. | |
| dc.format | pdf | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Journal of Insect Physiology | |
| dc.relation | Volume 56, Issue 9, Pages 1050-1057, September 2010 | |
| dc.rights | Elsevier Ltd. | |
| dc.subject | Fitness cost | |
| dc.subject | Cost mitigation | |
| dc.subject | Insecticide resistance | |
| dc.subject | Hydrolases | |
| dc.subject | Stored grains insects | |
| dc.subject | Enzyme kinetics | |
| dc.title | Modified α-amylase activity among insecticide-resistant and -susceptible strains of the maize weevil, Sitophilus zeamais | |
| dc.type | Artigo | |
