Effect of heat stress and feeding phosphorus levels on pig electron transport chain gene expression

dc.creatorWeller, M. M. D. C. A.
dc.creatorAlebrante, L.
dc.creatorCampos, P. H. R. F.
dc.creatorSaraiva, A.
dc.creatorSilva, B. A. N.
dc.creatorDonzele, J. L.
dc.creatorOliveira, R. F. M.
dc.creatorSilva, F. F.
dc.creatorGasparino, E.
dc.creatorLopes, P. S.
dc.creatorGuimarães, S. E. F.
dc.date2018-06-05T10:59:01Z
dc.date2018-06-05T10:59:01Z
dc.date2013-09-09
dc.date.accessioned2023-09-27T21:02:33Z
dc.date.available2023-09-27T21:02:33Z
dc.identifier1751732X
dc.identifierhttp://dx.doi.org/10.1017/S1751731113001535
dc.identifierhttp://www.locus.ufv.br/handle/123456789/19925
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8953397
dc.descriptionThe purpose of this study was to evaluate the effect of temperature and different levels of available phosphorus (aP) on the expression of nine genes encoding electron transport chain proteins in the Longissimus dorsi (LD) muscle of pigs. Two trials were carried out using 48 high-lean growth pigs from two different growth phases: from 15 to 30 kg (phase 1) and from 30 to 60 kg (phase 2). Pigs from growth phase 1 were fed with three different levels of dietary aP (0.107%, 0.321% or 0.535%) and submitted either to a thermoneutral (24°C and RH at 76%) or to a heat stress (34°C and RH at 70%) environment. Pigs from growth phase 2 were fed with three different levels of dietary aP (0.116%, 0.306% or 0.496%) and submitted either to a thermoneutral (22ºC and RH at 77%) or to a heat stress (32ºC and RH at 73%) environment. Heat stress decreased (P<0.001) average daily feed intake at both growth phases. At 24°C, pigs in phase 1 fed the 0.321% aP diet had greater average daily gain and feed conversion (P<0.05) than those fed the 0.107% or 0.535% while, at 34°C pigs fed the 0.535% aP had the best performance (P<0.05). Pigs from phase 2 fed the 0.306% aP had best performance in both thermal environments. Gene expression profile was analyzed by quantitative real-time polymerase chain reaction. Irrespective of growing phase, the expression of six genes was lower (P<0.05) at high temperature than at thermoneutrality. The lower expression of these genes under high temperatures evidences the effects of heat stress by decreasing oxidative metabolism, through adaptive physiological mechanisms in order to reduce heat production. In pigs from phase 1, six genes were differentially expressed across aP levels (P<0.05) in the thermoneutral and one gene in the heat stress. In pigs from phase 2, two genes were differentially expressed across aP levels (P<0.05) in both thermal environments. These data revealed strong evidence that phosphorus and thermal environments are key factors to regulate oxidative phosphorylation with direct implications on animal performance.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherAnimal
dc.relationv. 7, n. 12, p. 1985-1993, Dezembro 2013
dc.rightsOpen Access
dc.subjectNutrigenomics
dc.subjectOxidative phosphorylation
dc.subjectPig production
dc.subjectHeat stress and animal production
dc.subjectPhosphorous levels for pig nutrition
dc.titleEffect of heat stress and feeding phosphorus levels on pig electron transport chain gene expression
dc.typeArtigo


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