Evidence of illegitimate recombination between two pasteurellaceae plasmids resulting in a novel multi-resistance replicon, pM3362MDR, in Actinobacillus pleuropneumoniae

dc.creatorSilva, Giarlã Cunha da
dc.creatorLi, Yinghui
dc.creatorLi, Yanwen
dc.creatorRossi, Ciro C.
dc.creatorCrespo, Roberto Fernandez
dc.creatorWilliamson, Susanna M.
dc.creatorLangford, Paul R.
dc.creatorBazzolli, Denise Mara Soares
dc.creatorBossé, Janine T.
dc.date2019-03-11T16:33:58Z
dc.date2019-03-11T16:33:58Z
dc.date2018-10
dc.date.accessioned2023-09-27T21:01:38Z
dc.date.available2023-09-27T21:01:38Z
dc.identifier1664302X
dc.identifierhttps://doi.org/10.3389/fmicb.2018.02489
dc.identifierhttp://www.locus.ufv.br/handle/123456789/23849
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8953078
dc.descriptionEvidence of plasmids carrying the tetracycline resistance gene, tet(B), was found in the previously reported whole genome sequences of 14 United Kingdom, and 4 Brazilian, isolates of Actinobacillus pleuropneumoniae. Isolation and sequencing of selected plasmids, combined with comparative sequence analysis, indicated that the four Brazilian isolates all harbor plasmids that are nearly identical to pB1001, a plasmid previously found in Pasteurella multocida isolates from Spain. Of the United Kingdom isolates, 13/14 harbor plasmids that are (almost) identical to pTetHS016 from Haemophilus parasuis. The remaining United Kingdom isolate, MIDG3362, harbors a 12666 bp plasmid that shares extensive regions of similarity with pOV from P. multocida (which carries bla ROB−1 , sul2, and strAB genes), as well as with pTetHS016. The newly identified multi-resistance plasmid, pM3362MDR, appears to have arisen through illegitimate recombination of pTetHS016 into the stop codon of the truncated strB gene in a pOV-like plasmid. All of the tet(B)-carrying plasmids studied were capable of replicating in Escherichia coli, and predicted origins of replication were identified. A putative origin of transfer (oriT) sequence with similar secondary structure and a nic-site almost identical to that of RP4 was also identified in these plasmids, however, attempts to mobilize them from an RP4-encoding E. coli donor strain were not successful, indicating that specific conjugation machinery may be required.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers in Microbiology
dc.relationVolume 09, Article 2489, Pages 01- 09, October 2018
dc.rightsOpen Access
dc.subjectPlasmids
dc.subjectAntimicrobial resistance
dc.subjectTetracycline
dc.subjectRespiratory tract
dc.subjectPasteurellaceae
dc.titleEvidence of illegitimate recombination between two pasteurellaceae plasmids resulting in a novel multi-resistance replicon, pM3362MDR, in Actinobacillus pleuropneumoniae
dc.typeArtigo


Este ítem pertenece a la siguiente institución