Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on Pectoralis major muscle in chicken (Gallus gallus)

dc.creatorNascimento, Carlos S.
dc.creatorBarbosa, Leandro T.
dc.creatorBrito, Claudson
dc.creatorFernandes, Roberta P. M.
dc.creatorMann, Renata S.
dc.creatorPinto, Ana Paula G.
dc.creatorOliveira, Haniel C.
dc.creatorDodson, Mike V.
dc.creatorGuimarães, Simone E. F.
dc.creatorDuarte, Marcio S.
dc.date2017-10-23T13:26:07Z
dc.date2017-10-23T13:26:07Z
dc.date2015-05-28
dc.date.accessioned2023-09-27T20:58:13Z
dc.date.available2023-09-27T20:58:13Z
dc.identifier1932-6203
dc.identifierhttps://doi.org/10.1371/journal.pone.0127935
dc.identifierhttp://www.locus.ufv.br/handle/123456789/12269
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8951906
dc.descriptionThirteen reference genes were investigated to determine their stability to be used as a housekeeping in gene expression studies in skeletal muscle of chickens. Five different algorithms were used for ranking of reference genes and results suggested that individual rankings of the genes differed among them. The stability of the expression of reference genes were validated using samples obtained from the Pectoralis major muscle in chicken. Samples were obtained from chickens in different development periods post hatch and under different nutritional diets. For gene expression calculation the ΔΔCt approach was applied to compare relative expression of pairs of genes within each of 52 samples when normalized to mitochondrially encoded cytochrome c oxidase II (MT-CO2) target gene. Our findings showed that hydroxymethylbilane synthase (HMBS) and hypoxanthine phosphoribosyl transferase 1 (HPRT1) are the most stable reference genes while transferrin receptor (TFRC) and beta-2-microglobulin (B2M) ranked as the least stable genes in the Pectoralis major muscle of chickens. Moreover, our results revealed that HMBS and HPRT1 gene expression did not change due to dietary variations and thus it is recommended for accurate normalization of RT-qPCR data in chicken Pectoralis major muscle.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherPlos One
dc.relation10(5): e0127935
dc.rightsOpen Access
dc.subjectPectoralis major
dc.subjectGallus gallus
dc.subjectIdentification of Suitable
dc.titleIdentification of suitable reference genes for real time quantitative polymerase chain reaction assays on Pectoralis major muscle in chicken (Gallus gallus)
dc.typeArtigo


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