Rough virulent strain of Brucella ovis induces pro- and anti-inflammatory cytokines in reproductive tissues in experimentally infected rams

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorAntunes, João Marcelo Azevedo de Paula
dc.creatorAllendorf, Susan Dora
dc.creatorAppolinário, Camila Michele
dc.creatorCagnini, Didier Quevedo
dc.creatorFigueiredo, Paula Ripamonte
dc.creatorJúnior, José Buratini
dc.creatorBaños, Joaquin Vicente
dc.creatorKocan, Katherine M.
dc.creatorde la Fuente, José
dc.creatorMegid, Jane
dc.date2014-05-27T11:28:13Z
dc.date2016-10-25T18:42:54Z
dc.date2014-05-27T11:28:13Z
dc.date2016-10-25T18:42:54Z
dc.date2013-01-25
dc.date.accessioned2017-04-06T02:10:48Z
dc.date.available2017-04-06T02:10:48Z
dc.identifierVeterinary Microbiology, v. 161, n. 3-4, p. 339-343, 2013.
dc.identifier0378-1135
dc.identifier1873-2542
dc.identifierhttp://hdl.handle.net/11449/74422
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74422
dc.identifier10.1016/j.vetmic.2012.07.024
dc.identifierWOS:000312524400013
dc.identifier2-s2.0-84870392541
dc.identifierhttp://dx.doi.org/10.1016/j.vetmic.2012.07.024
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895188
dc.descriptionThe ovine brucellosis caused by Brucella ovis has tropism for reproductive tissues but until now the mechanism of bacterial persistence is not understood. Cytokine expression profiles were studied for 8 months in rams after being experimentally infected with the rough virulent strain of B. ovis (R- B. ovis) to study the pathogenesis of B. ovis and immune mechanism possibly associated to bacteria tropism and persistence. The messenger RNA (mRNA) expression levels of interleukin-1α (IL-1α), IL-1β, IL-6, IL-10, IL-12, interferon-γ (INF-γ) and tumour necrosis factor-α (TNF-α) cytokines were quantified by real-time quantitative RT-PCR (qRT-PCR) in reproductive tissues (epididymus, testicles, ampolae, vesicular glands and bulbourethral glands), and non-reproductive (liver, spleen and kidneys) tissues at 30, 60, 120 and 240 days post infection (dpi). During the acute phase of infection at 30. dpi, the host immune response was most notable demonstrating an up-regulation of several cytokines in reproductive tissues, including the epididymus (IL-6, IL-1β and IL-1α), testicles (INF-γ and IL-12), bulbourethral glands (IL-6 and TNF-α) and ampolae (INF-γ, IL-10, IL-1β and IL-1α). During the development of infection, cytokine gene expression levels decreased, providing evidence of immunosuppression and evidence of immune evasion that favoured persistence of chronic R- B. ovis infection. During the chronic phase of R- B. ovis infection (120 and 240. dpi), cytokine production was down-regulated in the epididymus (IL-1β and IL-1α), testicles (INF-γ and IL-12), and ampolae (INF-γ, IL-10, IL-1β and IL-1α), with the exception of the bulbourethral glands (IL-6 and TNF-α) and epididymus (IL-6); in these tissues, R- B. ovis infection resulted in up-regulation of the pro-inflammatory cytokine IL-6. Herein, we report cytokine expression profiles in tissues of rams experimentally infected with the rough strain of B. ovis, which are associated with bacterial persistence and macrophage activation. © 2012 Elsevier B.V.
dc.languageeng
dc.relationVeterinary Microbiology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBrucella ovis
dc.subjectBrucellosis
dc.subjectCytokines
dc.subjectGene expression
dc.subjectRam
dc.subjectRough strain
dc.subjectcytokine
dc.subjectgamma interferon
dc.subjectinterleukin 10
dc.subjectinterleukin 12
dc.subjectinterleukin 1alpha
dc.subjectinterleukin 1beta
dc.subjectinterleukin 6
dc.subjectmessenger RNA
dc.subjecttumor necrosis factor alpha
dc.subjectampolae
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectbacterial strain
dc.subjectbacterial virulence
dc.subjectbrucellosis
dc.subjectbulbourethral gland
dc.subjectcontrolled study
dc.subjectepididymis
dc.subjectexperimental infection
dc.subjectgene expression
dc.subjectgenital system
dc.subjectimmune deficiency
dc.subjectimmune evasion
dc.subjectimmune response
dc.subjectkidney
dc.subjectliver
dc.subjectnonhuman
dc.subjectprotein blood level
dc.subjectquantitative analysis
dc.subjectram (sheep)
dc.subjectreal time polymerase chain reaction
dc.subjectsheep disease
dc.subjectspleen
dc.subjecttestis
dc.subjecttropism
dc.subjectvesicular gland
dc.subjectAnimals
dc.subjectGenitalia, Male
dc.subjectInflammation
dc.subjectMacrophage Activation
dc.subjectMale
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectSheep
dc.subjectSheep Diseases
dc.subjectUp-Regulation
dc.subjectBacteria (microorganisms)
dc.subjectBrucella melitensis biovar Ovis
dc.subjectOvis
dc.titleRough virulent strain of Brucella ovis induces pro- and anti-inflammatory cytokines in reproductive tissues in experimentally infected rams
dc.typeOtro


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