Use of hemoglobin as alternative to peroxidases in cholesterol amperometric biosensors

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSouza, Tâmera T.L.
dc.creatorMoraes, Marli L.
dc.creatorFerreira, Marystela
dc.date2014-05-27T11:28:11Z
dc.date2016-10-25T18:42:48Z
dc.date2014-05-27T11:28:11Z
dc.date2016-10-25T18:42:48Z
dc.date2013-01-21
dc.date.accessioned2017-04-06T02:10:31Z
dc.date.available2017-04-06T02:10:31Z
dc.identifierSensors and Actuators, B: Chemical, v. 178, p. 101-106.
dc.identifier0925-4005
dc.identifierhttp://hdl.handle.net/11449/74407
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74407
dc.identifier10.1016/j.snb.2012.12.005
dc.identifierWOS:000315751100012
dc.identifier2-s2.0-84872342188
dc.identifierhttp://dx.doi.org/10.1016/j.snb.2012.12.005
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895173
dc.descriptionSophisticated molecular architectures can be produced with the layer-by-layer (LbL) method, which may combine distinct materials on the same film. In this study, we take advantage of this capability to produce cholesterol amperometric biosensors from LbL films containing hemoglobin (Hb) and cholesterol oxidase in addition to the polyelectrolytes poly(allylamine hydrochloride) (PAH) and poly(ethylene imine) (PEI). Following an optimization procedure, we found that an LbL film deposited onto ITO substrates, with the architecture ITO(PEI/Hb)5(PEI/COx)10, yielded a sensitivity of 93.4 μA μmol L-1 cm-2 for cholesterol incorporated into phospholipid liposomes, comparable to state-of-the-art biosensors. Hb acted as efficient electron mediator and did not suffer interference from phospholipids. Significantly, cholesterol could also be detected in real samples from chicken egg yolk, with no effects from potential interferents, including phospholipids. Taken together these results demonstrate the possible fabrication of low cost, easy-to-use cholesterol amperometric biosensors, whose sensitivity can be enhanced by further optimizing the molecular architectures of the LbL films. © 2012 Elsevier B.V.
dc.languageeng
dc.relationSensors and Actuators B: Chemical
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAmperometric measurements
dc.subjectBiosensor
dc.subjectCholesterol
dc.subjectEnzymes
dc.subjectHemoglobin
dc.subjectLbL film
dc.subjectAmperometric biosensors
dc.subjectChicken egg yolk
dc.subjectCholesterol oxidase
dc.subjectElectron mediator
dc.subjectInterferents
dc.subjectLayer-by-layer methods
dc.subjectLow costs
dc.subjectMolecular architecture
dc.subjectOptimization procedures
dc.subjectPhospholipid liposomes
dc.subjectPoly(allylamine hydrochloride)
dc.subjectPolyethyleneimine
dc.subjectReal samples
dc.subjectArchitecture
dc.subjectLiposomes
dc.subjectOptimization
dc.subjectPhospholipids
dc.subjectBiosensors
dc.titleUse of hemoglobin as alternative to peroxidases in cholesterol amperometric biosensors
dc.typeOtro


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