Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOliveira, M. L.
dc.creatorDuarte, J. M B
dc.date2014-05-27T11:28:10Z
dc.date2016-10-25T18:42:46Z
dc.date2014-05-27T11:28:10Z
dc.date2016-10-25T18:42:46Z
dc.date2013-01-16
dc.date.accessioned2017-04-06T02:10:21Z
dc.date.available2017-04-06T02:10:21Z
dc.identifierGenetics and Molecular Research, v. 12, n. 1, p. 44-52, 2013.
dc.identifier1676-5680
dc.identifierhttp://hdl.handle.net/11449/74388
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74388
dc.identifier10.4238/2013.January.16.8
dc.identifierWOS:000318864400006
dc.identifier2-s2.0-84872922539.pdf
dc.identifier2-s2.0-84872922539
dc.identifierhttp://dx.doi.org/10.4238/2013.January.16.8
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895155
dc.descriptionWe tried to amplify mitochondrial, microsatellite and amelogenin loci in DNA from fecal samples of a wild Mazama americana population. Fifty-two deer fecal samples were collected from a 600-ha seasonal semideciduous forest fragment in a subtropical region of Brazil (21°20′, 47°17′W), with the help of a detection dog; then, stored in ethanol and georeferenced. Among these samples 16 were classified as fresh and 36 as non-fresh. DNA was extracted using the QIAamp® DNA Stool Mini Kit. Mitochondrial loci were amplified in 49 of the 52 samples. Five microsatellite loci were amplified by PCR; success in amplification varied according to locus size and sample age. Successful amplifications were achieved in 10/16 of the fresh and in 13/36 of the non-fresh samples; a negative correlation (R = -0.82) was found between successful amplification and locus size. Amplification of the amelogenin locus was successful in 22 of the 52 samples. The difficulty of amplifying nuclear loci in DNA samples extractedfrom feces collected in the field was evident. Some methodological improvements, including collecting fresh samples, selecting primers for shorter loci and quantifying the extracted DNA by real-time PCR, are suggested to increase amplification success in future studies. © FUNPEC-RP.
dc.languageeng
dc.relationGenetics and Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCytochrome b
dc.subjectDetection dog
dc.subjectFecal DNA
dc.subjectMicrosatellite
dc.subjectMolecular ecology
dc.subjectamelogenin
dc.subjectcytochrome b
dc.subjectcytochrome c
dc.subjectmicrosatellite DNA
dc.subjectmitochondrial DNA
dc.subjectmolecular marker
dc.subject3' untranslated region
dc.subject5' untranslated region
dc.subjectage
dc.subjectanimal experiment
dc.subjectBrazil
dc.subjectcontrolled study
dc.subjectcytochrome b gene
dc.subjectdeer
dc.subjectDNA extraction
dc.subjectfeces analysis
dc.subjectfemale
dc.subjectgene locus
dc.subjectgenotype
dc.subjectgeographic distribution
dc.subjectmale
dc.subjectMazama americana
dc.subjectnonhuman
dc.subjectreal time polymerase chain reaction
dc.subjectsequence analysis
dc.subjectwild type
dc.titleAmplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)
dc.typeOtro


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