Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides

dc.creatorRezende, Sebastião T. de
dc.creatorMarques, Virgínia M.
dc.creatorTrevizano, Larissa M.
dc.creatorPassos, Flávia M. L.
dc.creatorOliveira, Maria G. A.
dc.creatorBemquerer, Marcelo P.
dc.creatorOliveira, Jamil S.
dc.creatorGuimarães, Valéria M.
dc.creatorViana, Pollyanna A.
dc.date2018-06-04T12:00:06Z
dc.date2018-06-04T12:00:06Z
dc.date2006-02-17
dc.date.accessioned2023-09-27T20:55:58Z
dc.date.available2023-09-27T20:55:58Z
dc.identifier15205118
dc.identifierhttp://dx.doi.org/10.1021/jf0526442
dc.identifierhttp://www.locus.ufv.br/handle/123456789/19902
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8951131
dc.descriptionRaffinose oligosaccharides (RO) are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. ROs are hydrolyzed by α-galactosidases that cleave α-1,6-linkages of α-galactoside residues. The objectives of this study were the purification and characterization of extracellular α-galactosidase from Debaryomyces hansenii UFV-1. The enzyme purified by gel filtration and anion exchange chromatographies presented an Mr value of 60 kDa and the N-terminal amino acid sequence YENGLNLVPQMGWN. The Km values for hydrolysis of pNPαGal, melibiose, stachyose, and raffinose were 0.30, 2.01, 9.66, and 16 mM, respectively. The α-galactosidase presented absolute specificity for galactose in the α-position, hydrolyzing pNPGal, stachyose, raffinose, melibiose, and polymers. The enzyme was noncompetitively inhibited by galactose (Ki = 2.7 mM) and melibiose (Ki = 1.2 mM). Enzyme treatments of soy milk for 4 h at 60 °C reduced the amounts of stachyose and raffinose by 100%.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherJournal of Agricultural and Food Chemistry
dc.relationv. 54, n. 6, p. 2385–2391, Fevereiro 2006
dc.rightsAmerican Chemical Society
dc.subjectα-Galactosidase
dc.subjectDebaryomyces hansenii UFV-1
dc.subjectRaffinose oligosaccharides
dc.subjectCharacterization
dc.subjectFlatulence
dc.titleExtracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides
dc.typeArtigo


Este ítem pertenece a la siguiente institución