Myenteric denervation in gastric carcinogenesis: Differential modulation of nitric oxide and annexin-A1

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPolli-Lopes, Ana Cláudia
dc.creatorEstofolete, Cássia F.
dc.creatorOliani, Sonia M.
dc.creatorZucoloto, Sérgio
dc.creatorCunha, Fernando Q.
dc.creatorGil, Cristiane D.
dc.date2014-05-27T11:27:33Z
dc.date2016-10-25T18:41:43Z
dc.date2014-05-27T11:27:33Z
dc.date2016-10-25T18:41:43Z
dc.date2013-01-01
dc.date.accessioned2017-04-06T02:08:36Z
dc.date.available2017-04-06T02:08:36Z
dc.identifierInternational Journal of Clinical and Experimental Pathology, v. 6, n. 1, p. 13-23, 2013.
dc.identifier1936-2625
dc.identifierhttp://hdl.handle.net/11449/74324
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74324
dc.identifierWOS:000313043300002
dc.identifier2-s2.0-84870472766.pdf
dc.identifier2-s2.0-84870472766
dc.identifierhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515986/
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895092
dc.descriptionThis study evaluated the properties of endogenous nitric oxide synthases (NOS) and annexin-A1 (ANXA1) and determined how they can be exploited in the N-methyl-N-nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinogenesis and myenteric denervation model. Male Wistar rats were treated with MNNG and/or aminoguanidine (AG) for 20 weeks. In another set of experiments, rats with nondenervated and denervated stomachs were treated with MNNG or water for 28 weeks. Fragments of the pyloric region were processed for histopathology, NOS activity, and immunohistochemistry to explore the activity and expression of constitutive (cNOS) and inducible (iNOS) NO synthase and their relationship with annexin-A1 (ANXA1) expression. NO inhibition by AG increased the percentage of animals with adenocarcinomas (~29%) compared with the untreated MNNG group (~4%). Myenteric denervation did not alter NOS activity. cNOS activity was significantly greater in nondernervated and denervated stomachs with or without lesions (P<0.001) than iNOS activity (P<0.01), as confirmed by immunohistochemistry. Further, cNOS activity in normal stomachs and outside the lesion area was considerably higher than inside it (P<0.01). By densitometric analysis of nondenervated and denervated stomachs, ANXA1 expression was modulated in epithelial and inflammatory cells (mast cells and neutrophils), wherein significant alterations were induced by lesion development and myenteric denervation. In conclusion, NO protects against the development of gastric adenocarcinomas. The pattern of ANXA1 expression was not associated with NOS activity or expression, suggesting that NO and ANXA1 act in gastric tumors in disparate pathways.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.relationInternational Journal of Clinical and Experimental Pathology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBenzalkonium chloride
dc.subjectImmunohistochemistry
dc.subjectInflammation
dc.subjectLipocortin-1
dc.subjectN-methyl-N-nitro-N-nitrosoguanidine
dc.subjectNitric oxide synthase
dc.subjectbenzalkonium chloride
dc.subjectlipocortin 1
dc.subjectnitric oxide
dc.subjectnitric oxide synthase
dc.subjectanimal
dc.subjectdenervation
dc.subjectimmunohistochemistry
dc.subjectinflammation
dc.subjectinnervation
dc.subjectlipocortin-1
dc.subjectmale
dc.subjectmetabolism
dc.subjectmyenteric plexus
dc.subjectpathology
dc.subjectphysiology
dc.subjectrat
dc.subjectstomach
dc.subjectstomach tumor
dc.subjectWistar rat
dc.subjectAnimals
dc.subjectAnnexin A1
dc.subjectDenervation
dc.subjectMale
dc.subjectMyenteric Plexus
dc.subjectNitric Oxide
dc.subjectNitric Oxide Synthase
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectStomach
dc.subjectStomach Neoplasms
dc.titleMyenteric denervation in gastric carcinogenesis: Differential modulation of nitric oxide and annexin-A1
dc.typeOtro


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