Restricted sugar uptake by sugar-induced internalization of the yeast lactose/galactose permease Lac12

dc.creatorRigamonte, Tatiana A.
dc.creatorSilveira, Wendel B.
dc.creatorFietto, Luciano G.
dc.creatorCastro, Ieso M.
dc.creatorBreunig, Karin D.
dc.creatorPassos, Flavia M.L.
dc.date2017-11-28T10:50:29Z
dc.date2017-11-28T10:50:29Z
dc.date2011-01-19
dc.date.accessioned2023-09-27T20:54:21Z
dc.date.available2023-09-27T20:54:21Z
dc.identifier15671364
dc.identifierhttps://doi.org/10.1111/j.1567-1364.2010.00709.x
dc.identifierhttp://www.locus.ufv.br/handle/123456789/13870
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8950571
dc.descriptionKluyveromyces lactis Lac12 permease mediates lactose and low-affinity galactose transports. In this study we investigated the effects of carbon sources on internalization of Lac12 using a LAC12–GFP fusion construct. When galactose- or lactose-grown cells are shifted to a fresh sugar medium, Lac12–GFP is removed from the plasma membrane and is localized intracellularly. Surprisingly, either galactose or lactose in the new media caused the internalization, and cells responded differently to these two sugars. Our results reveal that this process is dependent on sugar species and also sugar concentration. Lac12–GFP internalization causes reduction of [C14]lactose uptake rates and also occurs in a Klsnf1 mutant strain; it is therefore independent of KlSnf1 activity. We suggest that glucose-6-phosphate is the intracellular signal, as internalization was induced by 2-deoxyglucose, and inhibition of phosphoglucomutase by lithium prevented galactose- but not lactose- or glucose-induced internalization. Lac12–GFP internalization was not triggered by 6-deoxyglucose, and was irreversible in the absence of protein synthesis.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherFEMS Yeast Research
dc.relationVolume 11, Issue 3, Pages 243–251, May 2011
dc.rightsOpen Access
dc.subjectKluyveromyces lactis
dc.subjectLactose permease
dc.subjectLac12
dc.subjectPost-translational control
dc.subjectCatabolite inactivation
dc.subjectSugar transport
dc.titleRestricted sugar uptake by sugar-induced internalization of the yeast lactose/galactose permease Lac12
dc.typeArtigo


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