Comparative study of the behavior of p53 immunoexpression in smoking associated lesions: Reinke's edema and laryngeal carcinoma

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMóz, Luis Eduardo Silva
dc.creatorDomingues, Maria Aparecida Custódio
dc.creatorCastilho, Emanuel Celice
dc.creatorBranco, Anete
dc.creatorMartins, Regina Helena Garcia
dc.date2014-05-27T11:27:32Z
dc.date2016-10-25T18:41:38Z
dc.date2014-05-27T11:27:32Z
dc.date2016-10-25T18:41:38Z
dc.date2013-01-01
dc.date.accessioned2017-04-06T02:08:15Z
dc.date.available2017-04-06T02:08:15Z
dc.identifierInhalation Toxicology, v. 25, n. 1, p. 17-20, 2013.
dc.identifier0895-8378
dc.identifier1091-7691
dc.identifierhttp://hdl.handle.net/11449/74286
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74286
dc.identifier10.3109/08958378.2012.751142
dc.identifierWOS:000313285800003
dc.identifier2-s2.0-84872038163
dc.identifierhttp://dx.doi.org/10.3109/08958378.2012.751142
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895054
dc.descriptionObjective: To assess the behavior of the immunoexpression of protein p53 in Reinke's edema and laryngeal squamous cell carcinoma. Study design: retrospective. Methods: we recovered the histological paraffin blocks of patients who were subjected to Reinke's edema and laryngeal squamous cell carcinoma surgery in 2000-2011. The paraffin blocks were cut into 3-μm sections; the specimens were prepared in silanized slides (one slide for each paraffin block) and subjected to immunohistochemical reaction according to the Avidin Biotin Peroxidase method. Monoclonal primary anti-p53 antibodies were used at 1:50 dilution. Slides were examined under a light microscope at different magnitudes and results were interpreted based on the degree of brown staining in the nuclei of epithelial cells and in the extent of the fragment by using a semi-quantitative score from 0 to 3. Results: 67 slides of Reinke's edema and 60 slides of laryngeal squamous cell carcinoma were included. Scores 2 and 3 for staining of the nuclei of epithelial cells were recorded for 46 slides of Reinke's edema (68.65%) and for 57 slides of laryngeal squamous cell carcinoma (95%). As to the extent of the fragment, scores 2 and 3 were recorded for 74% slides of Reinke's edema and for 95% slides of carcinomas. Conclusion: the positive immunoexpression for protein p53, positive in 95% carcinomas and 74% Reinke's edemas, makes us aware of the possible preneoplastic condition of the latter lesion. Further studies are needed to identify and reveal the genetic changes that lead to these results. © 2013 Informa Healthcare USA, Inc.
dc.languageeng
dc.relationInhalation Toxicology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectImmunohistochemistry
dc.subjectLaryngeal squamous cell carcinoma
dc.subjectLarynx
dc.subjectP53
dc.subjectReinke's edema
dc.subjectmonoclonal antibody
dc.subjectprotein p53
dc.subjectadult
dc.subjectaged
dc.subjectcomparative study
dc.subjectepithelium cell
dc.subjectfemale
dc.subjecthuman
dc.subjectimmunohistochemistry
dc.subjectlarynx carcinoma
dc.subjectlarynx edema
dc.subjectlarynx injury
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectreinke edema
dc.subjectretrospective study
dc.subjectscoring system
dc.subjectsmoking
dc.subjectsquamous cell carcinoma
dc.subjectAdult
dc.subjectAged
dc.subjectCarcinoma, Squamous Cell
dc.subjectFemale
dc.subjectHumans
dc.subjectLaryngeal Edema
dc.subjectLaryngeal Mucosa
dc.subjectLaryngeal Neoplasms
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectPrecancerous Conditions
dc.subjectRetrospective Studies
dc.subjectSmoking
dc.subjectTumor Suppressor Protein p53
dc.titleComparative study of the behavior of p53 immunoexpression in smoking associated lesions: Reinke's edema and laryngeal carcinoma
dc.typeOtro


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