dc.creatorAlves, P. S.
dc.creatorRocha, M. S.
dc.date2018-08-27T17:54:50Z
dc.date2018-08-27T17:54:50Z
dc.date2012-03-21
dc.date.accessioned2023-09-27T20:54:10Z
dc.date.available2023-09-27T20:54:10Z
dc.identifier14320649
dc.identifierhttps://doi.org/10.1007/s00340-012-4938-4
dc.identifierhttp://www.locus.ufv.br/handle/123456789/21450
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8950501
dc.descriptionWe present a simple method to calibrate optical tweezers by using only videomicroscopy to measure the position autocorrelation function of the trapped bead in the potential well of the tweezers. To accomplish this task, we use a high-speed camera with ∼500 fps (frames per second), which provides a precise measurement of the relaxation time of the bead Brownian fluctuations. We also study the variation of the trap stiffness as a function of some parameters of interest such as the laser power and the distance from the bead center to the microscope coverslip, showing that the presented method returns precise results.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherApplied Physics B
dc.relationv. 107, n. 2, p. 375– 378, may 2012
dc.rightsSpringer-Verlag
dc.subjectOptical Tweezer
dc.subjectCMOS Camera
dc.subjectOptical Force
dc.subjectShort Measurement Time
dc.subjectBead Height
dc.titleVideomicroscopy calibration of optical tweezers by position autocorrelation function analysis
dc.typeArtigo


Este ítem pertenece a la siguiente institución